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首页> 外文期刊>American Journal of Physiology >Localization of ClC-2 Cl- channels in rabbit gastric mucosa.
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Localization of ClC-2 Cl- channels in rabbit gastric mucosa.

机译：ClC-2 Cl-通道在兔胃粘膜中的定位。

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摘要

HCl secretion across the parietal cell apical secretory membrane involves the H+-K+-ATPase, the ClC-2 Cl- channel, and a K+ channel. In the present study, the cellular and subcellular distribution of ClC-2 mRNA and protein was determined in the rabbit gastric mucosa and in isolated gastric glands. ClC-2 mRNA was localized to parietal cells by in situ hybridization and by direct in situ RT-PCR. By immunoperoxidase microscopy, ClC-2 protein was concentrated in parietal cells. Immunofluorescent confocal microscopy suggested that the ClC-2 was localized to the secretory canalicular membrane of stimulated parietal cells and to intracellular structures of resting parietal cells. Immunogold electron microscopy confirmed that ClC-2 is in the secretory canalicular membrane of stimulated cells and in tubulovesicles of resting parietal cells. These findings, together with previous functional characterization of the native and recombinant channel, strongly indicate that ClC-2 is the Cl- channel, which together with the H+-K+-ATPase and a K+ channel, results in HCl secretion across the parietal cell secretory membrane.
机译：整个壁细胞顶分泌膜的HCl分泌涉及H + -K + -ATPase，ClC-2 Cl-通道和K +通道。在本研究中，测定了兔胃粘膜和分离的胃腺中ClC-2 mRNA和蛋白的细胞和亚细胞分布。通过原位杂交和直接原位RT-PCR将ClC-2 mRNA定位于壁细胞。通过免疫过氧化物酶显微镜检查，将ClC-2蛋白浓缩在壁细胞中。免疫荧光共聚焦显微镜表明，ClC-2定位于受刺激壁细胞的分泌性小管膜和静息壁细胞的细胞内结构。免疫金电子显微镜证实，ClC-2位于受刺激细胞的分泌性小管膜和静息壁细胞的微管小泡中。这些发现以及以前对天然和重组通道的功能表征，强烈表明ClC-2是Cl-通道，与H + -K + -ATPase和K +通道一起，导致整个壁细胞分泌的HCl分泌。膜。

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