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首页> 外文期刊>American Journal of Physiology >Extracellular nucleotides regulate cellular functions of podocytes in culture.
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Extracellular nucleotides regulate cellular functions of podocytes in culture.

机译：细胞外核苷酸调节培养中足细胞的细胞功能。

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Extracellular nucleotides are assumed to be important regulators of glomerular functions. This study characterizes purinergic receptors in podocytes. The effects of purinergic agonists on electrophysiological properties and the intracellular free Ca(2+) concentration of differentiated podocytes were examined with the patch-clamp and fura 2 fluorescence techniques. mRNA expression of purinergic receptors was investigated by RT-PCR. Purinergic agonists depolarized podocytes. Purinergic agonists similarly increased intracellular free Ca(2+) concentration of podocytes. The rank order of potency of various nucleotides on membrane voltage and free cytosolic calcium concentration was UTP approximately UDP > [adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S)] > ATP > 2-methylthioadenosine 5'-triphosphate (2-MeS-ATP) > 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate (BzATP) > ADP-beta-S. alpha,beta-Me-ATP was without effect. In the presence of UTP, BzATP did not cause an additional depolarization of podocytes. Incubation of cells with ATP or BzATP did not induce lactate dehydrogenase release. In RT-PCR studies, mRNAs of the P2Y(1), P2Y(2), P2Y(6), and P2X(7) receptors were detected within glomeruli and podocytes. The data indicate that extracellular nucleotides modulate podocyte function mainly by an activation of both P2Y(2) and P2Y(6) receptors.
机译：假定细胞外核苷酸是肾小球功能的重要调节剂。这项研究的特征是足细胞中的嘌呤能受体。嘌呤激动剂对电生理特性和分化的足细胞胞内游离Ca（2+）浓度的影响用膜片钳和呋喃2荧光技术进行了检查。通过RT-PCR研究嘌呤能受体的mRNA表达。嘌呤能激动剂使足细胞去极化。嘌呤激动剂类似地增加足细胞的细胞内游离Ca（2+）浓度。各种核苷酸对膜电压和游离胞质钙浓度的效价排序为：UTP约为UDP> [腺苷5'-O-（3-硫代三磷酸）（ATP-γ-S）]> ATP> 2-甲基硫代腺苷5'-三磷酸（2-MeS-ATP）> 2'-和3'-O-（4-苯甲酰基苯甲酰基）-腺苷5'-三磷酸（BzATP）> ADP-beta-S。 α，β-Me-ATP没有作用。在UTP存在下，BzATP不会引起足细胞的额外去极化。用ATP或BzATP孵育细胞不会诱导乳酸脱氢酶释放。在RT-PCR研究中，在肾小球和足细胞内检测到P2Y（1），P2Y（2），P2Y（6）和P2X（7）受体的mRNA。数据表明，胞外核苷酸主要通过激活P2Y（2）和P2Y（6）受体来调节足细胞功能。

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