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首页> 外文期刊>American Journal of Physiology >Localization of prostaglandin E(2) EP2 and EP4 receptors in the rat kidney.
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Localization of prostaglandin E(2) EP2 and EP4 receptors in the rat kidney.

机译：大鼠肾脏中前列腺素E（2）EP2和EP4受体的定位。

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摘要

We investigated the localization of cAMP-coupled prostaglandin E(2) EP2 and EP4 receptor expression in the rat kidney. EP2 mRNA was restricted to the outer and inner medulla in rat kidney, as determined by RNase protection assay. RT-PCR analysis of microdissected resistance vessels and nephron segments showed EP2 expression in descending thin limb of Henle's loop (DTL) and in vasa recta of the outer medulla. The EP4 receptor was expressed in distal convoluted tubule (DCT) and cortical collecting duct (CCD) in preglomerular vessels, and in outer medullary vasa recta. Butaprost, an EP2 receptor-selective agonist, dose dependently raised cAMP levels in microdissected DTL and outer medullary vasa recta specimens but had no effect in EP2-negative outer medullary collecting duct segments. Dietary salt intake did not alter EP2 expression in the kidney medulla. These results suggest that PGE(2) may act in the resistance vessels and in the DTL and DCT-CCD segments as a paracrine, cAMP-dependent regulator of vascular resistance and tubular transport, respectively.
机译：我们调查了大鼠肾脏中cAMP耦合前列腺素E（2）EP2和EP4受体表达的定位。如RNase保护试验所确定，EP2 mRNA仅局限于大鼠肾脏的外部和内部髓质。显微切割的阻力血管和肾单位的RT-PCR分析显示，EP2在Henle's Loop（DTL）的下肢细小腿和髓质外血管直肠中表达。 EP4受体在肾小球前血管的远曲曲小管（DCT）和皮质收集管（CCD）中以及在髓外血管直肠中表达。 Butaprost，一种EP2受体选择性激动剂，在显微解剖的DTL和髓外静脉直肠样本中剂量依赖性地提高了cAMP的水平，但对EP2阴性的髓外收集管段没有影响。饮食中盐的摄入量不会改变肾髓质中EP2的表达。这些结果表明，PGE（2）可能在阻力血管中以及在DTL和DCT-CCD段中分别作为旁分泌，依赖cAMP的血管阻力和肾小管转运调节剂。

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