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首页> 外文期刊>American Journal of Physiology >X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice.
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X-ray microanalysis of airway surface liquid collected in cystic fibrosis mice.

机译：囊性纤维化小鼠中收集的气道表面液体的X射线显微分析。

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摘要

The airway surface liquid (ASL) that lines the airway surface epithelium plays a major role in airway antibacterial defense and mucociliary transport efficiency, two key factors in cystic fibrosis (CF) disease. A major difficulty is to collect ASL in native conditions without stimulation or alteration of the underlying airway epithelium. Using a cryoprobe specifically adapted to collect native ASL from the tracheal mouse surface, we analyzed by X-ray microanalysis the complete ASL and plasma ion content in Cftr(tm1Hgu)/Cftr(tm1Hgu) mice compared with that in control littermates. ASL ion content from eight Cftr(tm1Hgu)/Cftr(tm1Hgu) mice and eight control littermates did not appear significantly different. The mean (+/-SE) concentrations were 2,352 +/- 367 and 2,058 +/- 401 mmol/kg dry weight for Na, 1,659 +/- 272 and 1,448 +/- 281 mmol/kg dry weight for Cl, 357 +/- 57 and 337 +/- 38 mmol/kg dry weight for S, 1,066 +/- 220 and 787 +/- 182 mmol/kg dry weight for K, 400 +/- 82 and 301 +/- 58 mmol/kg dry weight for Ca, 105 +/- 31 and 105 +/- 20 mmol/kg dry weight for Mg, 33 +/- 15 and 29 +/- 9 mmol/kg dry weight for P in non-CF and CF mice, respectively. This cryotechnique appears to be a promising technique for analyzing the complete elemental composition of native ASL in CF and non-CF tissues.
机译：衬在气道表面上皮上的气道表面液体（ASL）在气道抗菌防御和粘膜纤毛运输效率中起着重要作用，这是囊性纤维化（CF）疾病的两个关键因素。一个主要的困难是在自然条件下收集ASL，而不会刺激或改变潜在的气道上皮。使用专门用于从气管小鼠表面收集天然ASL的冷冻探针，我们通过X射线显微分析与对照同窝仔相比，对Cftr（tm1Hgu）/ Cftr（tm1Hgu）小鼠的完整ASL和血浆离子含量进行了分析。八只Cftr（tm1Hgu）/ Cftr（tm1Hgu）小鼠和八只对照同窝仔小鼠的ASL离子含量没有显着差异。 Na的平均（+/- SE）浓度分别为2,352 +/- 367和2,058 +/- 401 mmol / kg干重，Cl，357 +的平均浓度为1,659 +/- 272和1,448 +/- 281 mmol / kg干重/-S的干重为57和337 +/- 38 mmol / kg，K的干重为1,066 +/- 220和787 +/- 182 mmol / kg，K的干重为400 +/- 82和301 +/- 58 mmol / kg在非CF和CF小鼠中，Ca的干重，Mg的干重105 +/- 31和105 +/- 20 mmol / kg，P的干重，M的干重为33 +/- 15和29 +/- 9 mmol / kg，分别。该冷冻技术似乎是用于分析CF和非CF组织中天然ASL完整元素组成的有前途的技术。

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