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首页> 外文期刊>American Journal of Physiology >Muc1 mucins on the cell surface are adhesion sites for Pseudomonas aeruginosa.
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Muc1 mucins on the cell surface are adhesion sites for Pseudomonas aeruginosa.

机译：细胞表面上的Muc1粘蛋白是铜绿假单胞菌的粘附位点。

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摘要

Recently, we cloned and characterized a full-length cDNA of the hamster Muc1 gene, the expression of which appears to be associated with secretory cell differentiation (Park HR, Hyun SW, and Kim KC. Am J Respir Cell Mol Biol 15: 237-244, 1996). The role of Muc1 mucins in the airway, however, is unknown. In this study, we investigated whether cell surface mucins are adhesion sites for Pseudomonas aeruginosa. Chinese hamster ovary (CHO) cells not normally expressing Muc1 mucin were stably transfected with the hamster Muc1 cDNA, and binding to P. aeruginosa was examined. Our results showed that 1) stably transfected CHO cells expressed both Muc1 mRNA and Muc1 mucins based on Northern and Western blot analyses, 2) Muc1 mucins present on the cell surface were degraded by neutrophil elastase, and 3) expression of Muc1 mucins on the cell surface resulted in a significant increase in adhesion of P. aeruginosa that was completely abolished by either proteolytic cleavage with neutrophil elastase or deletion of the extracellular domain by mutation. We conclude that Muc1 mucins expressed on the surface of CHO cells serve as adhesion sites for P. aeruginosa, suggesting a possible role for these glycoproteins in the early stage of airway infection and providing a model system for studying epithelial cell responses to bacterial adhesion that leads to airway inflammation in general and cystic fibrosis in particular.
机译：最近，我们克隆并鉴定了仓鼠Muc1基因的全长cDNA，其表达似乎与分泌细胞分化有关（Park HR，Hyun SW和Kim KC。Am J Respir Cell Mol Biol 15：237- 244，1996）。然而，Muc1粘蛋白在气道中的作用尚不清楚。在这项研究中，我们调查了细胞表面粘蛋白是否是铜绿假单胞菌的粘附位点。用仓鼠Muc1 cDNA稳定转染正常不表达Muc1粘蛋白的中国仓鼠卵巢（CHO）细胞，并检查其与铜绿假单胞菌的结合。我们的结果表明，1）稳定转染的CHO细胞根据Northern和Western印迹分析同时表达Muc1 mRNA和Muc1黏蛋白，2）存在于细胞表面的Muc1黏蛋白被中性粒细胞弹性蛋白酶降解，以及3）Muc1黏蛋白在细胞上的表达表面导致铜绿假单胞菌粘附力的显着增加，这可以通过用嗜中性粒细胞弹性蛋白酶的蛋白水解切割或通过突变缺失细胞外结构域而完全消除。我们得出的结论是，CHO细胞表面表达的Muc1粘蛋白充当铜绿假单胞菌的粘附位点，表明这些糖蛋白在气道感染的早期阶段可能发挥作用，并为研究上皮细胞对细菌粘附的反应提供了模型系统一般对气道炎症，尤其是囊性纤维化。

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