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首页> 外文期刊>American Journal of Physiology >Caffeine stores and dopamine differentially require Ca(2+) channels in goldfish somatotropes.
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Caffeine stores and dopamine differentially require Ca(2+) channels in goldfish somatotropes.

机译：咖啡因存储和多巴胺不同地需要金鱼生长激素中的Ca（2+）通道。

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摘要

The regulation of growth hormone (GH) secretion by intracellular Ca(2+) stores was studied in dissociated goldfish somatotropes. We characterized a caffeine-activated intracellular store that had been shown to mediate GH release in response to gonadotropin-releasing hormone. The peak response of caffeine stimulation was reduced by approximately 28% by 100 microM ryanodine in a use-dependent manner suggesting that the first 10 min of GH release is partially mediated by a caffeine-activated ryanodine receptor. The temporal sensitivities of caffeine- and dopamine-evoked GH release to blockade of Cd(2+)-sensitive Ca(2+) channels were compared. We demonstrated that the initial phase of dopamine-evoked release was dependent on Ca(2+) channels, whereas the initial phase of caffeine-evoked release was sensitive only to pretreatment blockade. This would suggest that the maintenance of one class of caffeine-activated intracellular stores requires entry of Ca(2+) through Cd(2+)-sensitive Ca(2+) channels. This differential temporal requirement for Ca(2+) channels in Ca(2+) signaling may be a mechanism to segregate intracellular signaling pathways of multiple neuroendocrine regulators in the teleost pituitary.
机译：生长激素（GH）分泌的细胞内Ca（2+）存储的调节在离解的金鱼生长激素中进行了研究。我们表征了咖啡因激活的细胞内存储，已被证明可调节促性腺激素释放激素对GH的释放。咖啡因刺激的峰值响应被100 microM ryanodine依赖于使用而减少了约28％，这表明GH释放的前10分钟部分由咖啡因激活的ryanodine受体介导。比较了咖啡因和多巴胺引起的GH释放对Cd（2+）敏感的Ca（2+）通道的阻断的时间敏感性。我们证明，多巴胺引起的释放的初始阶段取决于Ca（2+）通道，而咖啡因引起的释放的初始阶段仅对预处理阻滞敏感。这表明一类咖啡因激活的细胞内存储的维护需要通过Cd（2+）敏感的Ca（2+）通道进入Ca（2+）。 Ca（2+）信号中的Ca（2+）通道的这种不同的时间要求可能是分离硬骨脑垂体中多个神经内分泌调节剂的细胞内信号传导途径的机制。

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