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首页> 外文期刊>American Journal of Physiology >Surfactant protein interactions with neutral and acidic phospholipid films.
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Surfactant protein interactions with neutral and acidic phospholipid films.

机译：表面活性剂蛋白与中性和酸性磷脂膜的相互作用。

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The captive bubble tensiometer was employed to study interactions of phospholipid (PL) mixtures of dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) at 50 microg/ml with physiological levels of the surfactant protein (SP) A SP-B, and SP-C alone and in combination at 37 degrees C. All surfactant proteins enhanced lipid adsorption to equilibrium surface tension (gamma), with SP-C being most effective. Kinetics were consistent with the presence of two adsorption phases. Under the conditions employed, SP-A did not affect the rate of film formation in the presence of SP-B or SP-C. Little difference in gamma(min) was observed between the acidic POPG and the neutral POPC systems with SP-B or SP-C with and without SP-A. However, gamma(max) was lower with the acidic POPG system during dynamic, but not during quasi-static, cycling. Considerably lower compression ratios were required to generate low gamma(min) values with SP-B than SP-C. DPPC-POPG-SP-B was superior to the neutral POPC-SP-B system. Although SP-A had little effect on film formation with SP-B, surface activity during compression was enhanced with both PL systems. In the presence of SP-C, lower compression ratios were required with the acidic system, and with this mixture, SP-A addition adversely affected surface activity. The results suggest specific interactions between SP-B and phosphatidylglycerol, and between SP-B and SP-A. These observations are consistent with the presence of a surface-associated surfactant reservoir which is involved in generating low gamma during film compression and lipid respreading during film expansion.
机译：捕获气泡张力计用于研究二棕榈酰基磷脂酰胆碱（DPPC）和1-棕榈酰基-2-油酰基-sn-甘油3-磷酸胆碱（POPC）或1-棕榈酰基-2-油酰基-sn-的磷脂（PL）混合物的相互作用浓度为50微克/毫升的甘油3- [磷酸-rac-（1-甘油）]（POPG），具有生理水平的表面活性剂蛋白（SP）A SP-B和SP-C，单独使用和在37摄氏度下联合使用所有表面活性剂蛋白均能增强脂质吸附至平衡表面张力（γ），其中SP-C最有效。动力学与两个吸附相的存在是一致的。在所采用的条件下，SP-A在存在SP-B或SP-C的情况下不影响成膜速率。在带有和不带有SP-A的SP-B或SP-C的酸性POPG和中性POPC系统之间，观察到的gamma（min）差异很小。但是，在动态过程中，酸性POPG系统的gamma（max）较低，而在准静态循环过程中则没有。与SP-C相比，使用SP-B产生低gamma（min）值所需的压缩率要低得多。 DPPC-POPG-SP-B优于中性POPC-SP-B系统。尽管SP-A对SP-B的成膜影响很小，但两种PL体系均提高了压缩过程中的表面活性。在存在SP-C的情况下，酸性系统需要较低的压缩比，对于这种混合物，SP-A的添加会对表面活性产生不利影响。结果表明SP-B和磷脂酰甘油之间以及SP-B和SP-A之间的特定相互作用。这些观察结果与表面相关的表面活性剂储库的存在是一致的，该表面储库涉及在膜压缩期间产生低γ和在膜膨胀期间产生脂质再扩散。

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