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首页> 外文期刊>American journal of rhinology >A sheep model for the study of biofilms in rhinosinusitis.
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A sheep model for the study of biofilms in rhinosinusitis.

机译：用于研究鼻-鼻窦炎生物膜的绵羊模型。

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BACKGROUND: Bacterial biofllms have been shown in chronic diseases such as cystic fibrosis, cholesteatoma, and otitis media with effusion. Recently, their detection on the mucosal tissue of sinusitis patients has implicated them in the pathogenesis of this condition. We present an animal model using sheep experimentally infected with Staphylococcus aureus to study the possible association between biofilm and sinusitis. METHODS: Twenty-four sheep underwent bilateral endoscopic sinus surgery to identify their frontal ostia. The frontal sinuses were treated in one of the following ways according to preoperative randomization: (1) ostium left patent, (2) ostium left patent and bacteria instilled, (3) ostium occluded, or (4) ostium occluded and bacteria instilled. The frontal mucosa was harvested at day 7 and examined for biofilm presence using confocal scanning laser microscopy (CSLM) as well as scanning electron microscopy (SEM) and transmission electron microscopy (TEM). RESULTS: All three modalities showed different rates of biofilm detection. Three-dimensional structures that could be interpreted as biofilms were documented in 86% (n = 36) of the sinuses analyzed using SEM. These structures were seen in all four study groups. The detection rate using the other two modalities was much lower with CSLM, showing biofilms in 48% (n = 20) and TEM in only 29% (n = 12) of the sinuses analyzed. Unlike SEM, these two modalities only detected bacterial biofilms in sinuses randomized to bacterial instillation. CONCLUSION: The demonstration of bacterial biofilms in this animal model of sinusitis further supports the hypotheses that biofilms may play a role in the pathogenesis of this condition. There is an obvious discrepancy in the sensitivity and specificity of biofilm detection using the three modalities mentioned. CSLM appears to be the most objective technique. The inherent flaws, sampling error, and subjectivity involved in SEM and TEM make these less reliable in documenting biofilm existence.
机译：背景：细菌生物膜已在慢性疾病中显示出来，例如囊性纤维化，胆脂瘤和积液性中耳炎。最近，他们在鼻窦炎患者的粘膜组织上的检测将他们牵连到这种病的发病机理中。我们提出了一种动物模型，使用实验性感染金黄色葡萄球菌的绵羊来研究生物膜和鼻窦炎之间的可能联系。方法：二十四只羊接受了双侧内窥镜鼻窦手术以鉴定其额叶口。根据术前随机分组，以下列方式之一治疗额窦：（1）左开口骨，（2）左开口骨，细菌滴注，（3）闭口，或（4）闭孔，滴注细菌。在第7天收获额叶粘膜，并使用共聚焦扫描激光显微镜（CSLM）以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查生物膜的存在。结果：这三种方式均显示出不同的生物膜检出率。使用SEM分析的鼻窦中有86％（n = 36）记录了可以解释为生物膜的三维结构。这些结构在所有四个研究组中均可见。使用CSLM的其他两种方法的检出率要低得多，显示所分析的鼻窦中有48％（n = 20）的生物膜，而TEM仅29％（n = 12）的TEM。与SEM不同，这两种方式仅检测随机滴入细菌的鼻窦中的细菌生物膜。结论：在这种鼻窦炎动物模型中细菌生物膜的演示进一步支持了生物膜可能在这种疾病的发病机理中起作用的假设。使用上述三种方法在生物膜检测的敏感性和特异性方面存在明显差异。 CSLM似乎是最客观的技术。 SEM和TEM固有的缺陷，采样误差和主观性使得这些在记录生物膜存在方面不那么可靠。

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