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首页> 外文期刊>Biotechnology Letters >Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics
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Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics

机译：基于荧光共振能量转移原理的蛋白质生物传感器，用于监测细胞动力学

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摘要

Genetically-coded,fluorescence resonance energy transfer (FRET)biosensors are widely used to study molecular events from single cells to whole organisms.They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues.In this review,we discuss the theoretical basis of FRET with a focus on key parameters responsible for designing FRET biosensors that have the highest sensitivity.Next,we discuss recent applications that are grouped into four common biosensor design patterns-inter-molecular FRET,intramolecular FRET,FRET from substrate cleavage and FRET using multiple colour fluorescent proteins.Lastly,we discuss recent progress in creating fluorescent proteins suitable for FRET purposes.Together these advances in the development of FRET biosensors are beginning to unravel the interconnected and intricate signalling processes as they are occurring in living cells and organisms.
机译：遗传编码的荧光共振能量转移（FRET）生物传感器被广泛用于研究从单细胞到整个生物的分子事件。由于它们具有自发荧光并且对细胞器和组织都具有特异性，因此它们在生物传感器中是独一无二的。讨论了FRET的理论基础，重点是负责设计灵敏度最高的FRET生物传感器的关键参数。接下来，我们讨论了最近的应用，这些应用分为四个常见的生物传感器设计模式-分子间FRET，分子内FRET，底物FRET使用多种颜色的荧光蛋白进行切割和FRET。和生物。

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