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首页> 外文期刊>American Journal of Veterinary Research >Effect of infection with bovine leukosis virus on lymphocyte proliferation and apoptosis in dairy cattle
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Effect of infection with bovine leukosis virus on lymphocyte proliferation and apoptosis in dairy cattle

机译：牛白血病病毒感染对奶牛淋巴细胞增殖和凋亡的影响

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Objective-To determine effects of infection with bovine leukosis virus (BLV) on lymphocyte proliferation and apoptosis in dairy cattle.Animals-27 adult Holstein cows.Procedures-Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from lactating Holstein cows seronegative for BLV (n = 9 cows), seropositive for BLV and aleukemic (aleukemic; 9), and seropositive for BLV and persistently lymphocytotic (PL; 9). Isolated PBMCs were assayed for mitogen-induced proliferation and were analyzed by means of flow cytometry. The PBMCs from a subset of each group were assayed for apoptosis, caspase-9 activity, and expression of selected genes related to apoptosis.Results-PL cows had significantly higher total lymphocyte counts and significantly lower proportions of T-lymphocyte populations than did BLV-negative and aleukemic cows. Both groups of BLV-infected cows had significantly higher proportions of B cells and major histocompatibility complex II-expressing cells than did BLV-negative cows. Proliferation with concanavalin A was significantly lower for PL cows, compared with proliferation for BLV-negative cows. Pokeweed mitogen-induced proliferation was significantly higher for aleukemic and PL cows than for BLV-negative cows. Gene expression of apoptosis-inhibitory proteins BCL2 and BCL2L1 was significantly higher for aleukemic cows and expression of BCL2 was significantly higher for PL cows than for BLV-negative cows.Conclusions and Clinical Relevance-Cattle infected with BLV had marked changes in PBMC populations accompanied by alterations in proliferation and apoptosis mechanisms. Because the relative distribution and function of lymphocyte populations are critical for immune competence, additional studies are needed to investigate the ability of BLV-infected cattle to respond to infectious challenge. (Am J Vet Res 201172:1059-1064)
机译：目的-确定感染牛白血病病毒（BLV）对奶牛淋巴细胞增殖和凋亡的影响。动物-成年荷斯坦奶牛27头。 BLV（n = 9头母牛），BLV和无白血病（aurekemic; 9）呈血清阳性; BLV和持续淋巴细胞性（PL; 9）呈血清阳性。分析分离的PBMC的有丝分裂原诱导的增殖，并通过流式细胞术进行分析。测定每组子集的PBMC的凋亡，caspase-9活性以及与凋亡相关的选定基因的表达。结果-与BLV-相比，PL奶牛的总淋巴细胞计数显着更高，而T淋巴细胞群的比例则明显更低。阴性和无牛。两组BLV感染的母牛比BLV阴性的母牛具有更高比例的B细胞和主要组织相容性复合物II表达细胞。与BLV阴性母牛的增殖相比，PL母牛的伴刀豆球蛋白A的增殖明显更低。商陆和PL母牛的商陆有丝分裂原诱导的增殖显着高于BLV阴性母牛。凋亡抑制蛋白BCL2和BCL2L1的基因表达在无氧奶牛中显着升高，在PL奶牛中BCL2的表达显着高于在BLV阴性奶牛中。增殖和凋亡机制的改变。由于淋巴细胞群的相对分布和功能对于免疫能力至关重要，因此需要进一步的研究来调查感染BLV的牛对传染性挑战的反应能力。 （Am J Vet Res 201172：1059-1064）

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