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首页> 外文期刊>American Journal of Veterinary Research >Effect of West Nile virus DNA-plasmid vaccination on response to live virus challenge in red-tailed hawks (Buteo jamaicensis)
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Effect of West Nile virus DNA-plasmid vaccination on response to live virus challenge in red-tailed hawks (Buteo jamaicensis)

机译：西尼罗河病毒DNA质粒疫苗接种对红尾鹰（Buteo jamaicensis）活病毒攻击反应的影响

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Objective-To evaluate the safety and efficacy of an experimental adjuvanted DNA-plasmid vaccine against West Nile virus (WNV) in red-tailed hawks (Buteo jamaicensis).Animals-19 permanently disabled but otherwise healthy red-tailed hawks of mixed ages and both sexes without detectable serum antibodies against WNV.Procedures-Hawks were injected IM with an experimental WNV DNA-plasmid vaccine in an aluminum-phosphate adjuvant (n = 14) or with the adjuvant only (control group; 5). All birds received 2 injections at a 3-week interval. Blood samples for serologic evaluation were collected before the first injection and 4 weeks after the second injection (day 0). At day 0, hawks were injected SC with live WNV. Pre- and postchallenge blood samples were collected at intervals for 14 days for assessment of viremia and antibody determination; oropharyngeal and clonal swabs were collected for assessment of viral shedding.Results-Vaccination was not associated with morbidity or deaths. Three of the vaccinated birds seroconverted after the second vaccine injection; all other birds seroconverted following the live virus injection. Vaccinated birds had significantly less severe viremia and shorter and less-intense shedding periods, compared with the control birds.Conclusions and Clinical Relevance-Use of the WNV DNA plasmid vaccine in red-tailed hawks was safe, and vaccination attenuated but did not eliminate both the viremia and the intensity of postchallenge shedding following live virus exposure. Further research is warranted to conclusively determine the efficacy of this vaccine preparation for protection of red-tailed hawks and other avian species against WNV-induced disease. (Am J Vet Res 201172:1065-1070)
机译：目的-评价针对西尾尼罗河病毒（WNV）的实验性佐剂DNA质粒疫苗在红尾鹰（Buteo jamaicensis）中的安全性和有效性。动物19永久残废，但其他年龄段健康的红尾鹰没有可检测到的针对WNV的血清抗体的女性。过程中，给霍克斯注射了磷酸铝佐剂（n = 14）或仅佐剂（对照组; 5）中的实验性WNV DNA质粒疫苗。所有的鸡在3周的间隔内接受2次注射。在第一次注射之前和第二次注射后4周（第0天）收集用于血清学评估的血样。在第0天，向雄鹰注射活WNV。攻击前和攻击后每隔14天收集一次血样，以评估病毒血症和确定抗体。收集口咽拭子和克隆拭子以评估病毒的脱落。结果疫苗接种与发病率或死亡无关。第二次注射疫苗后，其中三只已免疫的禽鸟发生了血清转化。注射活病毒后，所有其他鸟类均发生血清转化。与对照鸟类相比，接种疫苗的鸟类的严重病毒血症明显更少，脱落期更短，强度更低。结论和临床意义-在红尾鹰中使用WNV DNA质粒疫苗是安全的，疫苗接种减弱但不能消除两者活病毒暴露后的病毒血症和攻击后脱落的强度。有必要进行进一步的研究来最终确定这种疫苗制剂对红尾鹰和其他禽类免受WNV引起的疾病的保护作用。 （Am J Vet Res 201172：1065-1070）

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