...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Veterinary Research >Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa
【24h】

Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa

机译：尖顶连接蛋白在犬十二指肠和结肠黏膜中的表达和亚细胞定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective-To examine the expression and distribution of tight junction (TJ) and adherens junction (AM proteins in canine duodenal and colonic mucosa.Sample-Mucosa obtained from 4 healthy Beagles.Procedures-Biopsy specimens of the duodenum and colon were obtained via endoscopy from 4 healthy dogs. The expression patterns and subcelluar localization of claudin-1, -2, -3, -4, -5, -7, and -8; E-cadherin; and beta-catenin in the duodenum and colon were analyzed by use of immunoblotting and immunofluorescence microscopy.Results-In the duodenum, there was clear expression of claudin-3 and -5, E-cadherin, and beta-catenin proteins and weak expression of claudin-7 protein. In contrast, there was clear expression of claudin-2 and -3, E-cadherin, and beta-catenin proteins and weak expression of claudin-5 and -7 proteins in the colon, as determined by use of immunoblotting. As determined by the use of immunofluorescence microscopy, the duodenum and colon had staining for claudin-3 and -5, E-cadherin, and beta-catenin in the most apical region and staining for claudin-7 in the basolateral region. Staining for claudin-2 was also observed in the colon.Conclusions and Clinical Relevance-Information was provided about the expression patterns of TJ and AJ proteins in the duodenum and colon of clinically normal dogs. These results may provide valuable information for use in evaluating the importance of these TJ and AJ proteins in the pathogenesis of inflammatory bowel disease in dogs. (Am J Vet Res 2011;72:1046-1051)
机译：目的-检查紧密连接（TJ）和粘附连接（AM蛋白）在犬十二指肠和结肠粘膜中的表达和分布，从4​​只健康的比格犬（Beagles）中获得样品-粘膜（Mucosa）。 4只健康的狗通过分析十二指肠和结肠中claudin-1，-2，-3，-4，-5，-7和-8，E-钙粘蛋白和β-连环蛋白的表达模式和亚细胞定位。结果-在十二指肠中，claudin-3和-5，E-cadherin和β-catenin蛋白表达清晰，而claudin-7蛋白表达弱。免疫印迹法测定十二指肠十二指肠中claudin-2和-3，E-cadherin和β-catenin蛋白的表达以及claudin-5和-7蛋白在结肠中的弱表达。和结肠的claudin-3和-5，E-cadher染色β-catenin在最顶端区域，而claudin-7在基底外侧区域染色。在结肠中也观察到了claudin-2的染色。结论和临床相关性信息提供了TJ和AJ蛋白在临床正常犬十二指肠和结肠中的表达模式。这些结果可能提供有价值的信息，以用于评估这些TJ和AJ蛋白在犬炎症性肠病发病机理中的重要性。 （Am J Vet Res 2011; 72：1046-1051）

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号