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首页> 外文期刊>American Zoologist >Glucose homeostasis in the teleost fish tilapia: insights from brockmann body xenotransplantation studies
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Glucose homeostasis in the teleost fish tilapia: insights from brockmann body xenotransplantation studies

机译：硬骨鱼罗非鱼中的葡萄糖稳态：来自布罗克曼人体异种移植研究的见解

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Certain teleost fish have large anatomically discrete islet organs called Brockmann bodies (BBs). When transplanted into streptozotocin diabetic athymic nude mice, tilapia BBs provide long-term normoglycemia. This has afforded us the opportunity to examine tilapia islet in vivo function in a non-piscine environment and compare this with in vivo function in the donor species. As expected, fasting and non-fasting glycemic levels in long-term murine recipients of tilapia BBs were analogous to corresponding values in donor tilapia, but, surprisingly, tilapia BB grafts provided mammalian-like glucose tolerance profiles. Teleost fish, in general, are severely glucose intolerant. When glucose tolerance tests were performed in tilapia, the mean glucose disappearance rates were very Iow; however, diabetic nude mice hearing long-term tilapia BB grafts were extremely glucose responsive. This suggested a severe or absolute peripheral resistance to the glucostatic effects of insulin. Using Western blotting with polyclonal antibodies and then confirmed by Northern analysis, tilapia peripheral tissues appear to be devoid of GLUT-4, the insulin-sensitive glucose transporter responsible for the hypoglycemic effect of insulin in mammals, but not GLUT-I, the insulin independent glucose transporter. This may explain why tilapia, and possibly other teleost fish, are severely glucose intolerant after pharmacologic glucose-loading. Because tllapia do not tend to consume large quantities of glucose in the wild, it is not surprising that they have evolved without a mechanism to move glucose rapidly from the bloodstream into muscle and fat. Nevertheless, insulin still appears to play an important role in maintaining normoglycemia in tilapia; however, this is mostly likely a result of its effect on glucose uptake in the liver. We also present comparative data on tilapia beta cell function, quantification of islet cell numbers and types, islet products, insulin gene structure and expression, and beta cell sensitivity to the diabetogenic drug streptozotocin.
机译：某些硬骨鱼类具有称为Brockmann体（BBs）的解剖学上较大的胰岛器官。罗非鱼BBs移植到链脲佐菌素糖尿病无胸腺裸鼠中后，可提供长期的正常血糖。这为我们提供了在非鱼类环境中检查罗非鱼胰岛体内功能并将其与供体物种体内功能进行比较的机会。如预期的那样，长期罗非鱼BBs小鼠的空腹和非空腹血糖水平类似于供体罗非鱼的相应值，但令人惊讶的是，罗非鱼BB移植物提供了哺乳动物样的葡萄糖耐量曲线。通常，硬骨鱼严重不耐葡萄糖。当在罗非鱼中进行葡萄糖耐量试验时，平均葡萄糖消失率非常低。但是，长期罗非鱼BB移植的糖尿病裸鼠对葡萄糖的反应极为强烈。这表明对胰岛素的糖调节作用有严重或绝对的外周抵抗。使用多克隆抗体进行蛋白质印迹，然后通过Northern分析证实，罗非鱼外周组织似乎不含负责胰岛素对哺乳动物降血糖作用的胰岛素敏感性葡萄糖转运蛋白GLUT-4，但不依赖于胰岛素非依赖性GLUT-1葡萄糖转运蛋白。这可以解释为什么罗非鱼以及可能的其他硬骨鱼在药理化葡萄糖负荷后严重不耐葡萄糖。由于罗非鱼在野外并不倾向于消耗大量的葡萄糖，因此它们进化而来的过程中并没有将葡萄糖快速从血液中转移到肌肉和脂肪中的机制也就不足为奇了。然而，胰岛素似乎在维持罗非鱼的正常血糖方面起着重要作用。然而，这很可能是其对肝脏葡萄糖摄取的影响的结果。我们还介绍了罗非鱼β细胞功能，胰岛细胞数量和类型，胰岛产物，胰岛素基因结构和表达以及β细胞对糖尿病药物链脲佐菌素的敏感性的比较数据。

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