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首页> 外文期刊>American-Eurasian Journal of Agricultural and Environmental Sciences >Evaluation of Guanidium Thiocyanate as a Chemical Mutagen on the Expression of Nitrate Reductase Gene (NR) in Chlorella Sp., NTMP04.
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Evaluation of Guanidium Thiocyanate as a Chemical Mutagen on the Expression of Nitrate Reductase Gene (NR) in Chlorella Sp., NTMP04.

机译：评价硫氰酸胍作为化学诱变剂对小球藻（NTMP04）中硝酸还原酶基因（NR）表达的影响。

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摘要

Nitrate is the predominant form of inorganic nitrogen utilized by higher plants, fungi and algae and its assimilation forms the major pathway for production of biologically useful nitrogen. Chlorella is a unicellular eukaryotic green alga, which offers a potentially useful system for the expression of hetrologous proteins. In the present study the microalgal samples were isolated from stagnant fresh water habitat (Tiruchirappalli, Tamil Nadu) and identified as Chlorella sp. NTMP04, Chlorella sp. NTMP05, Chlorella sp. NTMP06. Chemical mutation was adopted using guanidine thiocyanate as mutagen and the mutants were assessed in the specific media such as SGIl-NO, and SGII-NH4 for nitrate reductase (NR) gene regulation. A strain Chlorella sp, NTMP04 waschosen for the biochemical and molecular analysis to confirm NR gene mutation. The genomic DNA has extracted from the wild and mutant strains and PCR amplification of NR gene was done using degenegarative primers (NR-F and NR-R). The wild type of Chlorella sp NTMP04 showed the presence of NR gene, whereas mutant did not amplified the specific gene. The comparative analysis showed the evidence of chemical based mutation in the Chlorella sp., NTMP04.
机译：硝酸盐是高等植物，真菌和藻类所利用的无机氮的主要形式，其吸收形成了生产生物学上有用的氮的主要途径。小球藻是单细胞的真核绿藻，它为表达异源蛋白质提供了潜在的有用系统。在本研究中，微藻样品是从停滞的淡水生境（Tiruchirappalli，泰米尔纳德邦）中分离出来的，并鉴定为小球藻。 NTMP04，小球藻NTMP05，小球藻NTMP06。使用硫氰酸胍作为诱变剂进行化学突变，并在特定培养基如SGI1-NO和SGII-NH4中评估突变体的硝酸还原酶（NR）基因调控。选择了小球藻菌株NTMP04进行生化和分子分析，以证实NR基因突变。从野生和突变菌株中提取基因组DNA，并使用简并引物（NR-F和NR-R）对NR基因进行PCR扩增。小球藻NTMP04的野生型显示了NR基因的存在，而突变体未扩增该特定基因。对比分析显示了小球藻NTMP04中基于化学突变的证据。

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