...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Veterinary Research >Evaluation of infectivity of a canine lineage H3N8 influenza A virus in ponies and in primary equine respiratory epithelial cells
【24h】

Evaluation of infectivity of a canine lineage H3N8 influenza A virus in ponies and in primary equine respiratory epithelial cells

机译：评价犬系H3N8甲型流感病毒在小马和初级马呼吸道上皮细胞中的感染力

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective-To evaluate whether an equine-derived canine H3N8 influenza A virus was capable of infecting and transmitting disease to ponies.Animals-20 influenza virus seronegative 12- to 24-month-old ponies.Procedures-5 ponies were inoculated via aerosol exposure with 10(7) TCID50 of A/Canine/Wyoming/86033/07 virus (Ca/WY)/pony. A second group of 5 ponies (positive control group) was inoculated via aerosol exposure with a contemporary A/Eq/Colorado/10/07 virus (Eq/CO), and 4 sham-inoculated ponies served as a negative control group. To evaluate the potential for virus transmission, ponies (3/inoculation group) were introduced 2 days after aerosol exposure and housed with Ca/WY- and Eq/CO-inoculated ponies to serve as sentinel animals. Clinical signs, nasal virus shedding, and serologic responses to inoculation were monitored in all ponies for up to 21 days after viral inoculation. Growth and infection characteristics of viruses were examined by use of Madin-Darby canine kidney cells and primary equine and canine respiratory epithelial cells.Results-Ponies inoculated with Ca/WY had mild changes in clinical appearance, compared with results for Eq/CO-inoculated ponies. Additionally, Ca/WY inoculation induced significantly lower numbers for copies of the matrix gene in nasal secretions and lower systemic antibody responses in ponies than did Eq/CO inoculation. The Ca/WY isolate was not transmitted to sentinel ponies.Conclusions and Clinical Relevance-Inoculation of ponies with the canine H3N8 isolate resulted in mild clinical disease, minimal nasal virus shedding, and weak systemic antibody responses, compared with responses after inoculation with the equine H3N8 influenza isolate. These results suggested that Ca/WY has not maintained infectivity for ponies. (Am J Vet Res 2011;72:1071-1078)
机译：目的-评估马源性犬H3N8甲型流感病毒是否能感染小马并传播疾病;动物20流感病毒血清型12至24个月大的小马;程序5小马通过气溶胶暴露接种。 A /犬/怀俄明/ 86033/07病毒（Ca / WY）/小马的10（7）TCID50。第二组5个小马（阳性对照组）通过气雾暴露接种当代A / Eq / Colorado / 10/07病毒（Eq / CO），4个假接种小马用作阴性对照组。为了评估病毒传播的潜力，在暴露于气溶胶​​的两天后引入小马（3只/接种组），并以Ca / WY和Eq / CO接种的小马作为小动物。病毒接种后长达21天，监测所有小马的临床体征，鼻病毒脱落和对接种的血清学反应。通过使用Madin-Darby犬肾细胞，原代马和犬呼吸道上皮细胞检查了病毒的生长和感染特征。结果-接种Ca / WY的小马的临床外观较Eq / CO接种的小。小马。此外，与Eq / CO接种相比，Ca / WY接种诱导的鼻分泌物中基质基因拷贝数显着降低，小马中的全身抗体应答降低。 Ca / WY分离株未传播到前哨小马。结论和临床相关性-与马接种后的反应相比，用犬H3N8分离株接种小马导致临床疾病轻，鼻病毒脱落最少，全身抗体反应较弱。 H3N8流感隔离株。这些结果表明，Ca / WY并未保持小马的传染性。 （Am J Vet Res 2011; 72：1071-1078）

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号