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首页> 外文期刊>Amino acids >The intramolecular delta~(15)N of lysine responds to respiratory status in Paracoccus denitrificans
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The intramolecular delta~(15)N of lysine responds to respiratory status in Paracoccus denitrificans

机译：脱氮副球菌的赖氨酸分子内δ〜（15）N响应呼吸状态

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Presented here is the first experimental evidence that natural, intramolecular, isotope ratios are sensitive to physiological status, based on observations of intramolecular delta~(15)N of lysine in the mitochondrial mimic Paracoccus denitrificans. Paracoccus denitrificans, a versatile, gram-negative bacterium, was grown either aerobically or anaerobically on isotopically-characterized ammonium as sole cell-nitrogen source. Nitrogen isotope composition of the biomass with respect to source ammonium was triangle open N_(cell-NH_4) = delta~(15)N_(cell) - delta~(15)N_(NH_4) = -6.2 亇 1.2 per thousand for whole cells under aerobic respiration, whereas cells grown anaerobically produced no net fractionation (triangle open ~(15)N_(cell-NH_4) = -0.3 亇 0.23 per thousand). Fractionation of ~(15)N between protein nitrogen and total cell nitrogen increased during anaerobic respiration and suggests that residual nitrogen-containing compounds in bacterial cell membranes are isotopically lighter under anaerobic respiration. In aerobic cells, the lysine intramolecular difference between peptide and sidechain nitrogen is negligible, but in anaerobic cells was a remarkable triangle open ~(15)N_(p-s) = delta~(15)N_(peptide)-delta~(15)N_(sidechain) = +11.0 per thousand, driven predominantly by enrichment at the peptide N. Consideration of known lysine pathways suggests this to be likely due to enhanced synthesis of peptidoglycans in the anaerobic state. These data indicate that distinct pathway branching ratios associated with microbial respiration can be detected by natural intramolecular triangle open delta~(15)N measurements, and are the first in vivo observations of position-specific measurements of nitrogen isotope fractionation.
机译：基于线粒体反硝化副球菌中线粒体δ〜（15）N的分子内δ〜（15）N的观察，本文首次提出了自然的，分子内的同位素比率对生理状态敏感的实验证据。反硝化副球菌是一种多功能的革兰氏阴性细菌，在有同位素特征的铵盐上作为唯一的细胞氮源，需氧或厌氧生长。相对于源铵，生物质的氮同位素组成为三角形开放N_（cell-NH_4）=δ〜（15）N_（cell）-δ〜（15）N_（NH_4）= -6.2亇1.2 /每千个完整细胞在有氧呼吸下，厌氧生长的细胞没有产生净分离（三角形开口〜（15）N_（cell-NH_4）= -0.3亇0.23 /千）。厌氧呼吸过程中蛋白质氮与总细胞氮之间〜（15）N的分数增加，这表明细菌细胞膜中残留的含氮化合物在厌氧呼吸下同位素变轻。在需氧细胞中，肽和侧链氮之间的赖氨酸分子内差异可以忽略不计，但在厌氧细胞中，显着的三角形开口为〜（15）N_（ps）=δ〜（15）N_（肽）-δ〜（15）N_ （侧链）=千分之+11.0，主要由肽N处的富集驱动。考虑到已知的赖氨酸途径，这可能是由于厌氧状态下肽聚糖合成增强所致。这些数据表明，与微生物呼吸有关的独特的途径分支比可以通过自然的分子内三角开放δ〜（15）N测量来检测，并且是在体内首次观察到的氮同位素分馏的位置特异性测量。

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