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首页> 外文期刊>American journal of otolaryngology >Laser disruption and killing of methicillin-resistant Staphylococcus aureus biofilms.
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Laser disruption and killing of methicillin-resistant Staphylococcus aureus biofilms.

机译：激光打散和杀死耐甲氧西林的金黄色葡萄球菌生物膜。

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OBJECTIVE: The aim of the study was to study the efficacy of 2 different lasers in vitro, in disrupting biofilm and killing planktonic pathogenic bacteria. MATERIALS AND METHODS: Biofilms of a stable bioluminescent of Staphylococcus aureus Xen 31 were grown in a 96-well microtiter plate for 3 days. The study included 7 arms: (a) control; (b) ciprofloxacin (3 mg/L, the established minimum inhibitory concentration [MIC]) alone; (c) shock wave (SW) laser alone; (d) near-infrared (NIR) laser alone; (e) SW laser and ciprofloxacin; (f) SW and NIR lasers; (g) SW, NIR lasers, and ciprofloxacin. The results were evaluated with an in vivo imaging system (IVIS) biophotonic system (for live bacteria) and optical density (OD) for total bacteria. RESULTS: Without antibiotics, there was a 43% reduction in OD (P < .05) caused by the combination of SW and NIR suggesting that biofilm had been disrupted. There was an 88% reduction (P < .05) in live biofilm. Ciprofloxacin alone resulted in a decrease of 28% of total live cells (biofilm remaining attached) and 58% of biofilm cells (both P > .05). Ciprofloxacin in combination with SW and SW + NIR lasers caused a decrease of more than 60% in total live biomass and more than 80% of biofilm cells, which was significantly greater than ciprofloxacin alone (P < .05). CONCLUSIONS: We have demonstrated an effective nonpharmacologic treatment method for methicillin-resistant Staphylococcus aureus (MRSA) biofilm disruption and killing using 2 different lasers. The preferred treatment sequence is a SW laser disruption of biofilm followed by NIR laser illumination. Treatment optimization of biofilm is possible with the addition of ciprofloxacin in concentrations consistent with planktonic MIC.
机译：目的：本研究的目的是研究两种不同的激光在体外破坏生物膜和杀死浮游致病细菌的功效。材料与方法：稳定的金黄色葡萄球菌Xen 31生物发光的生物膜在96孔微量滴定板中生长3天。该研究包括7个方面：（a）对照; （b）单独使用环丙沙星（3 mg / L，确定的最低抑菌浓度[MIC]）; （c）仅冲击波（SW）激光; （d）仅近红外（NIR）激光; （e）短波激光和环丙沙星; （f）短波和近红外激光器; （g）SW，NIR激光和环丙沙星。使用体内成像系统（IVIS）生物光子系统（用于活细菌）和总细菌的光密度（OD）评估结果。结果：如果不使用抗生素，SW和NIR的结合导致OD降低43％（P <.05），表明生物膜已被破坏。活生物膜减少了88％（P <.05）。单独使用环丙沙星减少了28％的活细胞（保持附着的生物膜）和58％的生物膜细胞（均P> .05）。环丙沙星与SW和SW + NIR激光结合使用后，总活生物量减少了60％以上，生物膜细胞减少了80％以上，这明显大于单独使用环丙沙星的情况（P <.05）。结论：我们已经证明了一种有效的非药物治疗方法，可以使用2种不同的激光对甲氧西林耐药的金黄色葡萄球菌（MRSA）生物膜进行破坏和杀死。优选的处理顺序是先对生物膜进行SW激光破坏，再进行NIR激光照射。环丙沙星的浓度与浮游性MIC一致，可以优化生物膜的治疗效果。

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