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首页> 外文期刊>Amyotrophic lateral sclerosis eofficial publication of the World Federation of Neurology Research Group on Motor Neuron Diseases >No evidence of HIV pol gene in spinal cord tissues in sporadic ALS by real-time RT-PCR
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No evidence of HIV pol gene in spinal cord tissues in sporadic ALS by real-time RT-PCR

机译：实时RT-PCR未发现散发性ALS患者脊髓组织中的HIV pol基因

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摘要

An association of retroviruses and especially HIV has been reported in SALS, but CNS tissues have not been directly tested.To be valid, direct testing should be performed in light of the highly variable distribution of neuropathology from one patient to another and the degradable nature of RNA viruses. We aimed to seek direct evidence of retrovirus and especially HIV in SALS by real-time RT-PCR. We tested 20 SALS spinal cords in our repository specifically created for RNA preservation. We extracted RNA from anterior horns and surrounding tissue scrape from each sample and validated RNA quality. We indexed our search to neuropathologic degeneration and tested multiple areas in many of the nervous systems.We used realtime RT-PCR to a highly conserved region in the highly conserved pol gene and established the lowest limit of detection using a positive control. HIV-1 pol gene sequence was not detected in any of the 20 SALS nervous systems. Our assay was able to detect down to 60 viral copy numbers. In conclusion, we could not find direct evidence of retroviral/HIV activity in SALS CNS to support a relationship. However, our method could miss novel retroviruses with non-homology in the pol gene.
机译：在SALS中已报道了逆转录病毒特别是与HIV的关联，但尚未对中枢神经系统组织进行直接测试。为使之有效，应根据从一名患者到另一名患者的神经病理学高度变化的分布以及该患者的可降解性质进行直接测试。 RNA病毒。我们旨在通过实时RT-PCR寻找SALS中逆转录病毒尤其是HIV的直接证据。我们在专门用于RNA保藏的储存库中测试了20条SALS脊髓。我们从每个样品的前角和周围组织刮擦物中提取RNA，并验证了RNA的质量。我们对神经病理学退化进行了检索索引，并在许多神经系统中测试了多个区域。我们对高度保守的pol基因中的高度保守区域使用了实时RT-PCR，并使用阳性对照确定了最低检测限。在20个SALS神经系统中均未检测到HIV-1 pol基因序列。我们的测定能够检测到多达60个病毒拷贝数。总之，我们无法在SALS CNS中找到逆转录病毒/ HIV活性支持关系的直接证据。但是，我们的方法可能会错过pol基因中具有非同源性的新型逆转录病毒。

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