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首页> 外文期刊>BioMed research international >Evaluation of Insulin Medium or Chondrogenic Medium on Proliferation and Chondrogenesis of ATDC5 Cells
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Evaluation of Insulin Medium or Chondrogenic Medium on Proliferation and Chondrogenesis of ATDC5 Cells

机译：胰岛素培养基或软骨形成培养基对ATDC5细胞增殖和软骨形成的评估

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摘要

Background. The ATDC5 cell line is regarded as an excellent cell model for chondrogenesis. In most studies with ATDC5 cells, insulin medium (IM) was used to induce chondrogenesis while chondrogenic medium (CM), which was usually applied in chondrogenesis of mesenchymal stem cells (MSCs), was rarely used for ATDC5 cells. This study was mainly designed to investigate the effect of IM, CM, and growth medium (GM) on chondrogenesis of ATDC5 cells. Methods. ATDC5 cells were, respectively, cultured in IM, CM, and GM for a certain time. Then the proliferation and the chondrogenesis progress of cells in these groups were analyzed. Results. Compared with CM and GM, IM promoted the proliferation of cells significantly. CM was effective for enhancement of cartilage specific markers, while IM induced the cells to express endochondral ossification related genes. Although GAG deposition per cell in CM group was significantly higher than that in IM and GM groups, the total GAG contents in IM group were the most. Conclusion. This study demonstrated that CM focused on induction of chondrogenic differentiation while IM was in favor of promoting proliferation and expression of endochondral ossification related genes. Combinational use of these two media would be more beneficial to bone/cartilage repair.
机译：背景。 ATDC5细胞系被认为是软骨形成的优秀细胞模型。在大多数使用ATDC5细胞的研究中，胰岛素培养基（IM）用于诱导软骨生成，而通常用于间充质干细胞（MSCs）软骨生成的软骨生成培养基（CM）很少用于ATDC5细胞。这项研究的主要目的是研究IM，CM和生长培养基（GM）对ATDC5细胞软骨形成的影响。方法。将ATDC5细胞分别在IM，CM和GM中培养一定时间。然后分析了这些组中细胞的增殖和软骨形成过程。结果。与CM和GM相比，IM显着促进了细胞的增殖。 CM有效增强软骨特异性标记，而IM诱导细胞表达软骨内骨化相关基因。尽管CM组的每细胞GAG沉积量显着高于IM和GM组，但IM组的总GAG含量最高。结论。这项研究表明CM专注于诱导软骨分化，而IM则有利于促进软骨内骨化相关基因的增殖和表达。这两种介质的组合使用将更有利于骨/软骨修复。

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