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首页> 外文期刊>Biochemistry >The First Position of a Codon Placed in the A Site of the Human 80S Ribosome Contacts Nucleotide C1696 of the 18S rRNA as Well as Proteins S2, S3, S3a, S30, and S15.
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The First Position of a Codon Placed in the A Site of the Human 80S Ribosome Contacts Nucleotide C1696 of the 18S rRNA as Well as Proteins S2, S3, S3a, S30, and S15.

机译：密码子的第一位置放置在人80s核糖体的部位接触18S rRNA的核苷酸C1696以及蛋白质S2，S3，S3A，S30和S15。

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摘要

Messenger RNA analogues (42-mers) containing a GAC codon (aspartic acid) in the middle of their sequence followed by a s(4)UGA stop codon were used to identify the components of the human ribosomal A site in direct contact with the photoactivatable 4-thiouridine (s(4)U) residue. We compared the behavior of the nonphased ribosome-mRNA complex, (-)tRNA(Asp), to the one of the phased complex, (+)tRNA(Asp), in the absence and in the presence of eRF1, the eukaryotic class 1 translation termination factor of human origin. The patterns of cross-links obtained for the three complexes were similar to those previously reported for rabbit ribosomes [Chavatte, L., et al. (2001) Eur. J. Biochem. 268, 2896-2904]. Cross-links involving proteins S2, S3, S3a, and S30 were poorly dependent on the presence of tRNA(Asp) and eRF1. Cross-linking to nucleotide C1696 of 18S rRNA occurred in all complexes, but its yield was at least two times higher in the phased complex with an empty A site than in the nonphased complex or when the A site was occupied by eRF1. In contrast, protein S15 cross-linked only in the phased complex in the absence of eRF1. The data obtained point to notable differences in organization of the decoding site between mammalian and prokaryotic ribosomes and to large internal mobility of the components of the tRNA (eRF1)-free A site.
机译：在其序列中间含有GAC密码子（天冬氨酸）的信使RNA类似物（42-MER），然后用作（4）uga止芯密码子，用于鉴定与光活化4的直接接触的人核糖体的组分 - 硫氨酸（S（4）U）残留物。将非核糖体-mRNA复合物（ - ）TRNA（ASP）的行为与序列复合物（+）TRNA（ASP）中的一种，在不存在和ERF1的情况下，真核课程1翻译终止因子人类来源。为三分三络合物获得的交联的模式与先前报道的兔核糖体的那些模式相似[Chavatte，L.等人。 （2001）欧元。 J. Biochem。 268,2896-2904]。涉及蛋白质S2，S3，S3A和S30的交联依赖于TRNA（ASP）和ERF1的存在差。与核苷酸C1696的交联在所有复合物中发生18°C1696，但其产率在序号的络合物中至少两倍高，并且空的络合物中的含量比在非相反的复合物中或者当时占据ERF1占据时的位点。相反，蛋白S15在不存在ERF1的情况下仅在相位络合物中交联。数据获得了哺乳动物和原核核糖体之间的解码部位的组织中的显着差异，以及TRNA（ERF1）的组分的大型内部迁移率 - 免于左右部位。

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