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首页> 外文期刊>Biochemistry >Statistical conformation of human plasma fibronectin.
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Statistical conformation of human plasma fibronectin.

机译：人血浆纤连蛋白的统计构型。

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摘要

Fibronectin is a multifunctional glycoprotein (molecular mass, M = 530 kg/mol) of the extra cellular matrix (ECM) having a major role in cell adhesion. In physiological conditions, the conformation of this protein still remains debated and controversial. Here, we present a set of results obtained by scattering experiments. In "native" conditions, the radius of gyration (R(g) = 15.3 +/- 0.3 nm) was determined by static light scattering as well as small-angle neutron scattering. The hydrodynamic radius (R(H) = 11.5 +/- 0.1 nm) was deduced from quasi-elastic light scattering measurements. These results imply a low internal concentration compared to that of usual globular proteins. This is also confirmed by the ratio R(H)/R(g) = 0. 75 +/- 0.02 consistent with a Gaussian chain, whereas R(H)/R(g) = 1. 3 for spherical shaped molecules. However, adding a denaturing agent (urea 8 M) increases R(g) by a factor 2. This means that fibronectin "native" chain is not either completely unfolded. The average shape of fibronectin conformation was also probed by small-angle neutron scattering performed for reverse scattering vector q(-)(1) smaller than R(g) (0.2 < q(-)(1) < 15 nm). The measured form factor is in complete agreement with the form factor of a random string of 56 beads of 5 nm diameter. It rules out the possibility of unfolded chain as well as globular structures. These results have structural and biological implications as far as ECM organization is concerned.
机译：纤连蛋白是多核蛋白（分子量，m = 530kg / mol）的额外细胞基质（ECM）在细胞粘附中具有主要作用。在生理条件下，这种蛋白质的构象仍然仍然是争论和争议。在这里，我们提出了一系列通过散射实验获得的结果。在“本机”条件下，通过静态光散射以及小角度散射来确定环绕半径（R（g）= 15.3 +/- 0.3nm）。从准弹性光散射测量中推导出水动力半径（R（H）= 11.5 +/- 0.1nm）。与通常的球状蛋白相比，这些结果意味着低内浓度。这也通过与高斯链一致的比率R（H）/ R（g）= 0.75 +/- 0.02确认，而r（h）/ r（g）= 1. 3用于球形分子。然而，添加变性剂（尿素8m）将R（g）增加因子2.这意味着纤连蛋白“天然”链并不完全展开。通过对R（ - ）（1）小于R（g）的反向散射载体Q（ - ）（1）进行的小角度中子散射探测纤连蛋白构象的平均形状。测量的形式因子与56个直径为566珠的随机串的形状因子完全一致。它缩写了展开链和球状结构的可能性。随着ECM组织所关注的，这些结果具有结构性和生物学意义。

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