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首页> 外文期刊>Biochemistry >Bypass of a psoralen DNA interstrand cross-link by DNA polymerases β, l, and κ in Vitro
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Bypass of a psoralen DNA interstrand cross-link by DNA polymerases β, l, and κ in Vitro

机译：通过DNA聚合酶β，L和κ旁路Psoralen DNA Interstrand交联链路

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摘要

Repair of DNA interstrand cross-links in mammalian cells involves several biochemically distinctive processes, including the release of one of the cross-linked strands and translesion DNA synthesis (TLS). In this report, we investigated the in vitro TLS activity of a psoralen DNA interstrand cross-link by three DNA repair polymerases, DNA polymerases β, κ, and l. DNA polymerase β is capable of bypassing a psoralen cross-link with a low efficiency. Cell extracts prepared from DNA polymerase β knockout mouse embryonic fibroblasts showed a reduced bypass activity of the psoralen cross-link, and purified DNA polymerase β restored the bypass activity. In addition, DNA polymerase l misincorporated thymine across the psoralen cross-link and DNA polymerase κ extended these mispaired primer ends, suggesting that DNA polymerase l may serve as an inserter and DNA polymerase κ may play a role as an extender in the repair of psoralen DNA interstrand cross-links. The results demonstrated here indicate that multiple DNA polymerases could participate in TLS steps in mammalian DNA interstrand cross-link repair.
机译：哺乳动物细胞中的DNA Interstrand的修复涉及几种生物化学上独特的方法，包括释放其中一种交联链和转锁性DNA合成（TLS）。在本报告中，我们研究了三个DNA修复聚合酶，DNA聚合酶β，κ和κ和κ和κ和κ和β的体外TLS的体外TLS活性。 DNA聚合酶β能够以低效率绕过牛头醛交联。由DNA聚合酶β敲除小鼠胚胎成纤维细胞制备的细胞提取物显示出副通路的旁路活性，纯化的DNA聚合酶β恢复旁路活性。此外，DNA聚合酶L超薄副掺入胸腺嘧啶的胸腺嘧啶延伸这些错误的底漆延伸，表明DNA聚合酶L可以作为插入器和DNA聚合酶κ可在维护修复中发挥作用作为增量剂的作用DNA Interstrand Cross-Links。这里证明的结果表明，多种DNA聚合酶可以参与哺乳动物DNA Interstrand Cross-Link修复中的TLS步骤。

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  • 来源
    《Biochemistry》 |2012年第44期|共8页
  • 作者

    Smith L.A.; Makarova A.V.; Samson L.; Thiesen K.E.; Dhar A.; Bessho T.;

  • 作者单位

    Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center Omaha NE 68198 United States;

    Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center Omaha NE 68198 United States;

    Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center Omaha NE 68198 United States;

    Nebraska Wesleyan University Lincoln NE 68504 United States;

    Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center Omaha NE 68198 United States;

    Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center Omaha NE 68198 United States;

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  • 正文语种 eng
  • 中图分类 生物化学;
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