Identification of the in Vivo Function of the High-Efficiency D-Mannonate Dehydratase in Caulobacter crescentus NA1000 from the Enolase Superfamily

Daniel J. Wichelecki; Dylan C. Graff; Nawar Al-Obaidi; Steven C. Almo; John A. Gerlt

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Identification of the in Vivo Function of the High-Efficiency D-Mannonate Dehydratase in Caulobacter crescentus NA1000 from the Enolase Superfamily

机译：鉴别烯型超家族中高效D-甘露盐脱水酶的体内功能

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The D-mannonate dehydratase (ManD) subgroup of the enolase superfamily contains members with varying catalytic activities (high-efficiency, lowefficiency, or no activity) that dehydrate D-mannonate and/or D-gluconate to 2-keto-3-deoxy-D-gluconate [Wichelecki, D. J., et al. (2014) Biochemistry 53, 2722?2731]. Despite extensive in vitro characterization, the in vivo physiological role of a ManD has yet to be established. In this study, we report the in vivo functional characterization of a high-efficiency ManD from Caulobacter crescentus NA1000 (UniProt entry B8GZZ7) by in vivo discovery of its essential role in D-glucuronate metabolism. This in vivo functional annotation may be extended to ～50 additional proteins.

机译：烯醇酶超家族的D-甘露甘露酯脱水酶（MARE）亚组含有不同催化活性（高效率，低屈证性或无活性）的构件，其将D-甘露盐和/或D-葡萄糖酸盐脱水至2-酮-3-氧化 - D-Gluconate [Wichelecki，DJ等人。（2014）生物化学53,2722？2731]。尽管体外大量表征，但尚未建立法国的体内生理作用。在本研究中，通过体内发现其在D-葡萄糖酸酯代谢中的基本作用中，通过在D-葡萄糖酸酯代谢中的基本作用中报告来自大效率的高效核心的体内功能表征Na1000（Uniprot进入B8GZZ7）。这在体内功能性注释中可以延伸至〜50个另外的蛋白质。

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来源
《Biochemistry》 |2014年第25期|共3页
作者
Daniel J. Wichelecki; Dylan C. Graff; Nawar Al-Obaidi; Steven C. Almo; John A. Gerlt;
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作者单位

Institute for Genomic Biology University of Illinois at Urbana-Champaign Urbana Illinois 61801 United States;

Department of Biochemistry Albert Einstein College of Medicine Bronx New York 10461 United States;

Department of Biochemistry Albert Einstein College of Medicine Bronx New York 10461 United States;

Department of Biochemistry Albert Einstein College of Medicine Bronx New York 10461 United States;

Institute for Genomic Biology University of Illinois at Urbana-Champaign Urbana Illinois 61801 United States;

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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
Identification; Vivo Function; High-Efficiency;

机译：识别;体内功能;高效率;

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专利

1. Identification of the in Vivo Function of the High-Efficiency D-Mannonate Dehydratase in Caulobacter crescentus NA1000 from the Enolase Superfamily [J] . Daniel J. Wichelecki, Dylan C. Graff, Nawar Al-Obaidi, Biochemistry . 2014,第25期

机译：鉴别烯型超家族中高效D-甘露盐脱水酶的体内功能
2. Discovery of Function in the Enolase Superfamily: D?Mannonate and D?Gluconate Dehydratases in the D?Mannonate Dehydratase Subgroup [J] . Daniel J. Wichelecki, Bryan M. Balthazor, Anthony C. Chau, Biochemistry . 2014,第16期

机译：烯醇酶超家族中功能的发现：D？甘露酸酯脱水酶亚组中的D？甘露酸酯和D？葡萄糖酸脱水酶
3. Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus [J] . Rahman Mohammad Mubinur, Andberg Martina, Koivula Anu, Acta crystallographica. Section F, Structural biology communications . 2016,第8期

机译：L- rairabiumsarum bv的L-武装醛脱水酶的结晶和X射线衍射分析。 Trifolii和来自Carromcercentus的D-三角脱水酸碱
4. The identification of novel enzymatic functions in the enolase superfamily. [D] . Rakus, John Frank. 2009

机译：烯醇酶超家族中新型酶功能的鉴定。
5. Identification of the in Vivo Functionof the High-Efficiency d-Mannonate Dehydratase in Caulobacter crescentus NA1000 from the Enolase Superfamily [O] . DanielJ. Wichelecki, Dylan C. Graff, Nawar Al-Obaidi, -1

机译：体内功能的识别烯醇酶超家族中新月形杆菌NA1000中高效d-甘露聚糖脱水酶的研究
6. Correction of the Caulobacter crescentus NA1000 genome annotation. [O] . Bert Ely, LaTia Etheredge Scott 2014

机译：校正Caulobacter crescentus Na1000基因组注释。

Identification of the in Vivo Function of the High-Efficiency D-Mannonate Dehydratase in Caulobacter crescentus NA1000 from the Enolase Superfamily

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