Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy

Hossein Heidari Torkabadi; Christopher R. Bethel; Krisztina M. Papp-Wallace; Piet A. J. de Boer; Robert A. Bonomo; Paul R. Carey

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Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy

机译：通过拉曼微型光谱检查在大肠杆菌细胞内吸毒和反应之后

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Raman microspectroscopy combined with Raman difference spectroscopy reveals the details of chemical reactions within bacterial cells. The method provides direct quantitative data on penetration of druglike molecules into Escherichia coli cells in situ along with the details of drug?target reactions. With this label-free technique, clavulanic acid and tazobactam can be observed as they penetrate into E. coli cells and subsequently inhibit β-lactamase enzymes produced within these cells. When E. coli cells contain a β-lactamase that forms a stable complex with an inhibitor, the Raman signature of the known enamine acyl?enzyme complex is detected. From Raman intensities it is facile to measure semiquantitatively the number of clavulanic acid molecules taken up by the lactamase-free cells during growth.

机译：拉曼微穴位与拉曼差异光谱合并揭示了细菌细胞内化学反应的细节。该方法提供了关于药物状分子的直接定量数据，原位与药物的细节一起进入大肠杆菌细胞。目标反应。通过这种无标签技术，可以观察到克拉维酸和塔扎酰胺，因为它们渗透到大肠杆菌细胞中，随后抑制在这些细胞内产生的β-内酰胺酶。当大肠杆菌细胞含有与抑制剂形成稳定复合物的β-内酰胺酶时，检测已知烯胺酰基α酶复合物的拉曼特征。从拉曼强度从半定量测量，半定量测量在生长过程中由内酰胺酶的细胞占用的克拉维酸分子的数量。

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《Biochemistry》 |2014年第25期|共9页
作者
Hossein Heidari Torkabadi; Christopher R. Bethel; Krisztina M. Papp-Wallace; Piet A. J. de Boer; Robert A. Bonomo; Paul R. Carey;
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作者单位

Departments of Chemistry Microbiology Case Western Reserve University Cleveland Ohio 44106 United States;

Research Service Louis Stokes Cleveland Veterans Affairs Medical Center Cleveland Ohio 44106 United States;

Research Service Louis Stokes Cleveland Veterans Affairs Medical Center Cleveland Ohio 44106 United States;

Molecular Biology and Microbiology Case Western Reserve University Cleveland Ohio 44106 United States;

Research Service Louis Stokes Cleveland Veterans Affairs Medical Center Cleveland Ohio 44106 United States;

Departments of Chemistry Microbiology Case Western Reserve University Cleveland Ohio 44106 United States;

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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
Following Drug Uptake; Reactions; inside Escherichia;

机译：吸毒后;反应;在大肠杆菌里面;

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1. Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy [J] . Hossein Heidari Torkabadi, Christopher R. Bethel, Krisztina M. Papp-Wallace, Biochemistry . 2014,第25期

机译：通过拉曼微型光谱检查在大肠杆菌细胞内吸毒和反应之后
2. Looking inside the box: Using Raman microspectroscopy to deconstruct microbial biomass stoichiometry one cell at a time [J] . Hall E.K., Singer G.A., P?lzl M., The ISME journal emultidisciplinary journal of microbial ecology . 2011,第2期

机译：在盒子里寻找：使用拉曼光谱法一次分解一个细胞的微生物生物量化学计量
3. Prediction of injured and dead inactivated Escherichia coli O157:H7 cells after heat and pulsed electric field treatment with attenuated total reflectance infrared microspectroscopy combined with multivariate analysis technique. [J] . Gelaw T. K., Espina L., Pagan R., Food and bioprocess technology . 2014,第7期

机译：衰减和全反射红外光谱结合多元分析技术预测加热和脉冲电场处理后受伤和死亡的灭活大肠杆菌O157：H7细胞。
4. Raman and SERS microspectroscopy on living cells: a promising tool toward cellular drug response and medical diagnosis [C] . Abdelilah Beljebbar, Univ. de Reims Champagne-Ardenne, Reims, Biomedical Applications of Raman Spectroscopy . 1999

机译：拉曼光谱和SERS光谱对活细胞的研究：细胞药物反应和医学诊断的有前途的工具
5. Raman-microscopy of individual cells and applications monitoring drug uptake. [D] . Matthaeus, Christian B. 2006

机译：单个细胞的拉曼显微镜和监控药物吸收的应用。
6. Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy [O] . Hossein Heidari Torkabadi, Christopher R. Bethel, Krisztina M. Papp-Wallace, -1

机译：拉曼光谱法研究大肠杆菌细胞内的药物吸收和反应
7. Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy [O] . Hossein Heidari Torkabadi, Christopher R. Bethel, Krisztina M. Papp-Wallace, 2014

机译：通过拉曼微穴位检查在大肠杆菌细胞内的吸毒和反应之后
8. "Studies on the nature of the genetic material transferred during conjugation in Escherichia coli." and "Genetic and biochemical studies on the cell-free formation of tryptophan synthetase in Escherichia coli." [R] . 1963

机译：“在大肠杆菌结合过程中转移的遗传物质的性质研究。”和“在大肠杆菌中无色素形成色氨酸合成酶的遗传和生化研究”。

Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy

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