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首页> 外文期刊>Biochemistry >Conserved Residues in the Extracellular Loops of Short-Wavelength Cone Visual Pigments
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Conserved Residues in the Extracellular Loops of Short-Wavelength Cone Visual Pigments

机译：短波锥视觉颜料细胞外环循环中的保守残留物

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摘要

The role of the extracellular loop region of a short-wavelength sensitive pigment, Xenopus violet cone opsin, is investigated via computational modeling, mutagenesis, and spectroscopy. The computational models predict a complex H-bonding network that stabilizes and connects the EC2-EC3 loop and the N-terminus. Mutations that are predicted to disrupt the H-bonding network are shown to produce visual pigments that do not stably bind chromophore and exhibit properties of a misfolded protein. The potential role of a disulfide bond between two conserved Cys residues, Cys105 in TM3 and Cys182 in EC2, is necessary for proper folding and trafficking in VCOP. Lastly, certain residues in the EC2 loop are predicted to stabilize the formation of two antiparallel β-strands joined by a hairpin turn, which interact with the chromophore via H-bonding or van der Waals interactions. Mutations of conserved residues result in a decrease in the level of chromophore binding. These results demonstrate that the extracellular loops are crucial for the formation of this cone visual pigment. Moreover, there are significant differences in the structure and function of this region in VCOP compared to that in rhodopsin.
机译：通过计算建模，诱变和光谱研究了短波长敏感颜料的细胞外环区域的短波敏感颜料的作用。计算模型预测复杂的H键合网络，其稳定并连接EC2-EC3环路和N末端。预计破坏H键合网络的突变显示出产生不稳定结合发色团的视觉颜料并表现出错配的蛋白质的性质。二硫键在两种保守的Cys残留物，Cys105在TM3和EC2中的CYS182中的潜在作用是适当的折叠和贩运VCOP所必需的。最后，预测EC2环中的某些残基以稳定通过发夹转弯连接的两个反平行β-股，其通过H键合或范德华相互作用与发色团相互作用。保守残余物的突变导致发色团结合水平降低。这些结果表明，细胞外环对于形成这种锥形视觉颜料至关重要。此外，与罗多双可林相比，VCOP中该区域的结构和功能存在显着差异。

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  • 来源
    《Biochemistry》 |2011年第32期|共11页
  • 作者

    Min-Hsuan Chen; Daniel J. Sandberg; Kunnel R. Babu; Jose Bubis; Arjun Surya; Lavoisier S. Ramos; Heidi J. Zapata; Jhenny F. Galan; Megan N. Sandberg; Robert R. Birge; Barry E. Knox;

  • 作者单位

    Department of Biochemistry and Molecular Biology and Department of Ophthalmology SUNY Upstate Medical University Syracuse New York 13210 United States;

    Department of Chemistry and Department of Molecular and Cell Biology University of Connecticut Storrs Connecticut 06269 United States;

    Department of Biochemistry and Molecular Biology and Department of Ophthalmology SUNY Upstate Medical University Syracuse New York 13210 United States;

    Departamento de Biologia Celular Universidad Simon Bolivar Caracas Venezuela;

    Department of Biochemistry and Molecular Biology and Department of Ophthalmology SUNY Upstate Medical University Syracuse New York 13210 United States;

    Department of Chemistry and Department of Molecular and Cell Biology University of Connecticut Storrs Connecticut 06269 United States;

    Department of Biochemistry and Molecular Biology and Department of Ophthalmology SUNY Upstate Medical University Syracuse New York 13210 United States;

    Department of Chemistry and Department of Molecular and Cell Biology University of Connecticut Storrs Connecticut 06269 United States;

    Department of Chemistry and Department of Molecular and Cell Biology University of Connecticut Storrs Connecticut 06269 United States;

    Department of Chemistry and Department of Molecular and Cell Biology University of Connecticut Storrs Connecticut 06269 United States;

    Department of Biochemistry and Molecular Biology and Department of Ophthalmology SUNY Upstate Medical University Syracuse New York 13210 United States;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    Residues; Loops; Pigments;

    机译：残留物;环;颜料;

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