Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation

Rauzan B.; McMichael E.; Cave R.; Sevcik L.R.; Ostrosky K.; Whitman E.; Stegemann R.; Sinclair A.L.; Serra M.J.; Deckert A.A.

首页> 外文期刊>Biochemistry >Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation

【24h】

Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation

机译：DNA，RNA和杂交双链体形成的动力学和热力学

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The rates of duplex formation for two octamers of DNA (5′ d-CACGGCTC/5′ d-GAGCCGTG and 5′ d-CACAGCAC/5′ d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, ΔH and ΔS, have been determined for the same sequences under the same buffer conditions using optical melting techniques. These data reveal a linear free energy relationship between the free energy of activation for denaturation and the change in free energy for formation of the duplexes. This relationship indicates that these duplex formation reactions occur through a common unstructured transition state that is more similar to the single strands in solution than to the ensuing duplex. In addition, these data confirm that the greater stability of RNA duplexes relative to that of homologous DNA and hybrid duplexes is controlled by the denaturation rate and not the duplex formation rate.

机译：DNA（5'D-Cacggccc / 5'D-Gagccgtg和5'D-Cacagcac / 5'D-GTGCTG），同源RNA和两组杂种中的双工形成的双链体形成速率在1M NaCl缓冲液中已经使用停止流动光谱测量。另外，使用光学熔化技术在相同的缓冲条件下已经确定了热力学参数，ΔH和ΔS。这些数据揭示了激活的自由能量与变性的可自由能量和用于形成双链体的自由能的变化之间的线性自由能关系。这种关系表明，这些双工形成反应通过常见的非结构化过渡状态发生，该转换状态与溶液中的单链更类似于与随后的双工相同。另外，这些数据证实，通过变性率而不是双工形成速率来控制相对于同源DNA和混合双链体的RNA双链体的更大稳定性。

著录项

来源
《Biochemistry》 |2013年第5期|共8页
作者
Rauzan B.; McMichael E.; Cave R.; Sevcik L.R.; Ostrosky K.; Whitman E.; Stegemann R.; Sinclair A.L.; Serra M.J.; Deckert A.A.;
展开▼
作者单位

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

Department of Chemistry Allegheny College 520 North Main Street Meadville PA 16335 United States;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词

相似文献

外文文献
中文文献
专利

1. Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation [J] . Rauzan B., McMichael E., Cave R., Biochemistry . 2013,第5期

机译：DNA，RNA和杂交双链体形成的动力学和热力学
2. Thermodynamic and Kinetic Characterization of Duplex Formation between 2′-O, 4′-C-Methylene-modified Oligoribonucleotides, DNA and RNA [J] . Ulla Christensen Bioscience Reports . 2007,第6期

机译：2'-O，4'-C-亚甲基修饰的寡核糖核苷酸，DNA和RNA之间双链体形成的热力学和动力学表征
3. Solution conformation of an RNA-DNA hybrid duplex containing a pyrimidine RNA strand and a purine DNA strand [J] . Hantz E., Ladam P., Le Moyec L., International Journal of Biological Macromolecules: Structure, Function and Interactions . 2001,第4期

机译：包含嘧啶RNA链和嘌呤DNA链的RNA-DNA杂合双链体的溶液构象
4. Photo-triggered double duplex DNA formation by ultrafast DNA photo-cross-linking [C] . Daisuke Maeda, Hayato Kawabata, Shigetaka Nakamura 日本化学会春季年会講演予稿集 . 2018

机译：由Ultrafast DNA Photo-Crons-Connting的光触发双双双链DNA形成
5. Nonenzymatic oligomerization of RNA by TNA templates, and, Isoguanine-modified thrombin aptamers, and, An antiparallel purine·purine DNA duplex, and, Synthesis and enzymatic polymerization of FNA on DNA templates, and, Thermodynamics and luminescence of novel metallo-DNA base-pairs. [D] . Heuberger, Benjamin David. 2007

机译：TNA模板，异鸟嘌呤修饰的凝血酶适体，反平行嘌呤·嘌呤DNA双链体以及FNA在DNA模板上的合成和酶促聚合，以及新型金属DNA碱基的热力学和发光，通过RNA的非酶促寡聚化对。
6. Conformational properties and thermodynamics of the RNA duplex r(CGCAAAUUUGCG)2: comparison with the DNA analogue d(CGCAAATTTGCG)2. [O] . M R Conte, G L Conn, T Brown, 1997

机译：RNA双链体r（CGCAAAUUUGCG）2的构象性质和热力学：与DNA类似物d（CGCAAATTTGCG）2的比较。
7. Crystal structure and conformation of a DNA–RNA hybrid duplex with a polypurine RNA strand: d(TTCTTBr5CTTC)–r(GAAGAAGAA) [O] . Xiong Yong, Sundaralingam Muttaiya 1998

机译：具有多嘌呤RNA链的DNA–RNA杂合双链体的晶体结构和构象：d（TTCTTBr5CTTC）–r（GAAGAAGAA）
8. Thermodynamics and Kinetics of DNA, RNA, and Hybrid Oligonucleotide Double-Strand Formation [R] . Nelson, J. W. 1982

机译：DNa，RNa和杂合寡核苷酸双链形成的热力学和动力学

Kinetics and thermodynamics of DNA, RNA, and hybrid duplex formation

摘要

著录项

相似文献

相关主题

期刊订阅