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首页> 外文期刊>Biochemistry >Effect of mutation on the dissociation and recombination dynamics of CO in transcriptional regulator CooA: A picosecond infrared transient absorption study
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Effect of mutation on the dissociation and recombination dynamics of CO in transcriptional regulator CooA: A picosecond infrared transient absorption study

机译：突变对转录调节剂COOA中CO解离和重组动力学的影响：PICOSECOND红外瞬态吸收研究

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摘要

The CO ligation process in a mutant (H77G) of CooA, the CO-sensing transcriptional regulator in Rhodospirillum rubrum, is studied with femtosecond time-resolved transient absorption spectroscopy in the mid-infrared region. Following photolyzing excitation, a transient bleach in the vibrational region of bound CO due to the CO photodissociation is detected. In contrast to the spectra of the wild-type ( WT) CooA, the transient bleach spectra of H77G CooA show a bimodal shape with peaks shifting to the lower frequency during spectral evolution. The CO recombination dynamics show single-exponential behavior, and the CO escaping yield is higher than that of the WT CooA. A reorientation process of CO relative to the heme plane during recombination is revealed by anisotropy measurements. These phenomena indicate changes in the protein response to the CO ligation and suggest an alteration to the CO environment caused by the mutation. On the basis of these results, the role of His77 in the CO-dependent activation of CooA and a possible activation mechanism involving collaborative movement of the heme and the amino residues at both sides of the heme plane are discussed.
机译：在中红外区域中的飞秒时间分离的瞬态吸收光谱研究了COOA突变体（H77g）的CO突变体（H77g）的COOA中的COOA中的COOA的CO结合过程。在光解激励之后，检测到由于CO光探测的结合CO的振动区域中的瞬态漂白剂。与野生型（WT）COOA的光谱相反，H77G COOA的瞬时漂白光谱显示了在光谱进化期间与较低频率移位的双峰形状。 CO重组动力学显示单指数行为，CO转义产量高于WT COOA的CO。通过各向异性测量揭示了在重组期间相对于血红素平面的CO的重新定向过程。这些现象表明对CO结扎的蛋白质反应的变化，并表明由突变引起的CO环境改变。在这些结果的基础上，讨论了HIS77在COOA的共同激活中的作用和涉及血红素平面两侧的血红素和氨基残基的协同运动的可能活化机制。

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