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首页> 外文期刊>Biochemistry >Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM
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Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM

机译：甲基杆菌热情雌激素MCM中BOB1突变体的生物化学活性

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摘要

Minichromosomal maintenance proteins (MCMs) are considered to be the replicative helicase. Methanobacterium thermoautotrophicum has a single MCM gene (mtMCM). The crystal structure of the mtMCM N-terminal region is a double hexamer. Structure-guided sequence alignment indicates a structural conservation of this fragment across archaeal and eukaryotic MCMs. The mtMCM structure was successfully used to analyze a Saccharomyces cerevisiae MCM5 mutant, called BOB], which contains a single residue change from Pro to Leu and bypasses a kinase normally required for initiation of DNA replication. A domain-push model was proposed to explain the BOB1 bypass activity. Here we investigate the effects of BOB] mutation on the biochemical activities of mtMCM. Surprisingly, the BOB1 mutation (P62L) had a major effect on the helicase activity but had no significant impact on DNA binding and ATPase activities. These results will contribute to a more detailed understanding of the BOB] bypass activity and other aspects of DNA replication control.
机译：小核糖体维持蛋白（MCMS）被认为是复制螺旋酶。 Methanobacterium ThermooTotrophicum具有单一MCM基因（MTMCM）。 MTMC N末端区域的晶体结构是双六聚体。结构引导的序列对齐表明跨古藻和真核生物MCM的这种片段的结构保护。 MTMCM结构已成功地分析酿酒酵母，称为BOB的酿酒酵母MCM5突变体，其含有从Pro到Leu的单个残基变化，并绕过通常需要进行DNA复制所需的激酶。提出了一个域名推送模型来解释Bob1旁路活动。在这里，我们研究了Bob]突变对MTMCM生化活性的影响。令人惊讶的是，Bob1突变（P62L）对螺旋酶活性具有重大影响，但对DNA结合和ATP酶活性没有显着影响。这些结果将有助于更详细地了解鲍勃旁路活动和DNA复制控制的其他方面。

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