RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments

Forget AL; Kudron MM; McGrew DA; Calmann MA; Schiffer CA; Knight KL

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RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments

机译：RECA二聚体用作用于组装活性核蛋白长丝的功能单元

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All RecA-like recombinase enzymes catalyze DNA strand exchange as elongated filaments on DNA. Despite numerous biochemical and structural studies of RecA and the related Rad51 and RadA proteins, the unit oligomer(s) responsible for nucleoprotein filament assembly and coordinated filament activity remains undefined. We have created a RecA fused dimer protein and show that it maintains in vivo DNA repair and LexA co-protease activities, as well as in Vitro ATPase and DNA strand exchange activities. Our results support the idea that dimeric RecA is an important functional unit both for assembly of nucleoprotein filaments and for their coordinated activity during the catalysis of homologous recombination.

机译：所有RECA样重组酶酶催化DNA STRAND交换作为DNA上的细长细丝。尽管对RECA和相关RAD51和RADA蛋白的许多生化和结构研究，但负责核蛋白丝组件的单位低聚物和协调长丝活性仍未确定。我们创建了一种融合二聚体蛋白，并表明它在体内DNA修复和Lexa共蛋白酶活动中，以及体外ATP酶和DNA链交流活动。我们的研究结果支持，二聚体Rega是用于组装核蛋白细丝的重要功能单元，以及在同源重组的催化期间进行协调活性。

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来源
《Biochemistry》 |2006年第45期|共6页
作者
Forget AL; Kudron MM; McGrew DA; Calmann MA; Schiffer CA; Knight KL;
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作者单位

Univ Massachusetts Sch Med Dept Biochem &

Mol Pharmacol Worcester MA 01605 USA;

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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
SINGLE-STRANDED-DNA; PROMOTED ATP HYDROLYSIS; HUMAN RAD51 PROTEIN; P-LOOP MOTIF; ESCHERICHIA-COLI; SELF-ASSOCIATION; KINETIC-ANALYSIS; BINDING; COMPLEXES; RECOMBINATION;

机译：单链DNA;促进ATP水解;人RAD51蛋白;P圈图案;埃斯克里希菊属植物;自我关联;动力学分析;结合;复合物;复合物;重组;重组;

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专利

1. RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments [J] . Forget AL, Kudron MM, McGrew DA, Biochemistry . 2006,第45期

机译：RECA二聚体用作用于组装活性核蛋白长丝的功能单元
2. DNA Binding, Coprotease, and Strand Exchange Activities of Mycobacterial RecA Proteins: Implications for Functional Diversity among RecA Nucleoprotein Filaments [J] . K. Neelakanteshwar Patil Pawan Singh and K. Muniyappa Biochemistry . 2011,第2期

机译：分枝杆菌RecA蛋白的DNA结合，蛋白酶和链交换活性：RecA核蛋白丝之间功能多样性的含义。
3. DNA Binding, Coprotease, and Strand Exchange Activities of Mycobacterial RecA Proteins: Implications for Functional Diversity among RecA Nucleoprotein Filaments [J] . Patil KN, Singh P, Muniyappa K Biochemistry . 2011,第2期

机译：分枝杆菌RecA蛋白的DNA结合，蛋白酶和链交换活性：RecA核蛋白丝之间功能多样性的影响。
4. SELF-ASSEMBLY VIA 'TWO-POINT' BINDING OF A ZINC PORPHYRIN DIMER TO A BIS PYRIDINE FUNCTIONALIZED FULLERENE; SINGLET EMISSION STUDIES [C] . Francis DSouza, Suresh Gadde, Melvin E. Zandler, Electrochemical Society Annual Meeting . 2005

机译：通过“两点”锌卟啉二聚体与双吡啶官能化富勒烯的结合自组装;单线发射研究
5. Comparative analysis of Dmc1 and Rad51 nucleoprotein filaments. [D] . Sheridan, Sean D. 2008

机译：Dmc1和Rad51核蛋白丝的比较分析。
6. RecA Dimers Serve as a Functional Unit for Assembly of Active Nucleoprotein Filaments [O] . Anthony L. Forget, Michelle M. Kudron, Dharia A. McGrew, -1

机译：RecA二聚体充当活性核蛋白丝组装的功能单元
7. RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments [O] . Forget, Anthony L., Kudron, Michelle M., McGrew, Dharia A., 2006

机译：Reca二聚体用作组装活性核蛋白丝的功能单元

RecA dimers serve as a functional unit for assembly of active nucleoprotein filaments

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