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首页> 外文期刊>Biochemistry >Large Disk Intermediate Precedes Formation of Apolipoprotein A-I-Dimyristoylphosphatidylcholine Small Disks
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Large Disk Intermediate Precedes Formation of Apolipoprotein A-I-Dimyristoylphosphatidylcholine Small Disks

机译：载脂蛋白A-I-Divyristoylpholine小盘形成的大盘中间体前面的形成。

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摘要

Small ~8.5 nm disks formed spontaneously when dimyristoylphosphatidylcholine (DMPC) large unilamellar vesicles (LUVs) were incubated with apolipoprotein A-I (apoA-I) (100:1 molar ratio). However, in a time course study, the transient production of ~11 nm large disks was detected and isolated by gel filtration. The intermediate large disks contained three apoA-I molecules and were stable over time; however, when additional apoA-I was added, they formed small disks containing two molecules of apoA-I. The reaction kinetics of apoA-I with DMPC LUVs was monitored by fluorescence resonance energy transfer, and two phases were observed, supporting the presence of the intermediate in the formation of small disks. The lipid dynamics of LUVs and disks were assayed, revealing the presence of sequestered lipid-protein domains upon apoA-I binding to DMPC LUVs. In addition, the lipids in the intermediate large disks were more constrained than those in the small disks. We propose that apoA-I binds with DMPC LUVs to form small lipid-protein domains on the LUV; then the domains are released to form large disks, which can mature in the presence of additional apoA-I to form small disks. Thus, the formation of small apoA-I lipid disks proceeds through the formation of a large disk intermediate.
机译：当Divyristoylphosphatidyline（DMPC）大的Unilamellar囊泡（LUV）与载脂蛋白A-I（apoA-1）（100：1摩尔比）温育时，自发地形成小〜8.5nm。然而，在时间课程研究中，通过凝胶过滤检测和分离〜11nm大盘的瞬时产生。中间大盘含有三种apoa-1分子，随着时间的推移稳定;然而，当添加额外的apoA-1时，它们形成了包含两个apoa-1分子的小盘。通过荧光共振能量转移监测具有DMPC LUV的APOA-1的反应动力学，观察到两相，支撑中间体在形成小盘中的存在。测定Luvs和盘的脂质动态，揭示了ApoA-1与DMPC Luvs结合时螯合脂质蛋白域的存在。此外，中间大盘中的脂质比小盘中的脂质更受约束。我们提出了apoa-i与DMPC Luvs结合，形成LUV上的小脂质蛋白域;然后域被释放以形成大磁盘，这在存在额外的apoa-i的情况下可以成熟以形成小盘。因此，小apoa-i脂质盘的形成通过形成大盘中间体。

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  • 来源
    《Biochemistry》 |2007年第21期|共9页
  • 作者

    Keng Zhu; Gregory Brubaker; Jonathan D. Smith;

  • 作者单位

    Departments of Cell Biology and Cardiovascular Medicine Cleveland Clinic and Department of Molecular Medicine Cleveland Clinic Lerner College of Medicine Case School of Medicine Cleveland Ohio 44195;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
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