...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Trehalose favors a cutinase compact intermediate off-folding pathway
【24h】

Trehalose favors a cutinase compact intermediate off-folding pathway

机译：海藻糖有利于Cutinase紧凑的中间折叠途径

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The folding of cutinase, an enzyme displaying lipolytic activity, has been studied in the presence of trehalose. Equilibrium unfolding data show that trehalose increases the free energy change between folded and unfolded states. Unfolding kinetics reveal the presence of an intermediate which is ca. 60% folded in terms of solvent exposure. Trehalose stabilizes this intermediate relative to the folded state. In contrast, the intermediate revealed by folding kinetics is more compact than the transition state, as shown by the positive slope observed at low denaturant concentration in the chevron plot, as well as the decrease in the observable rate constant for folding with the increase in trehalose concentration. This intermediate displays more than 50% of area buried from the solvent (relative to the native state) compared to around 40% for the transition state for folding and therefore appears to be off the folding pathway. Trehalose stabilizes and guanidine hydrochloride destabilizes this compact intermediate. Both unfolding and folding kinetics show that compact conformational states are stabilized by trehalose, in agreement with current models on the effect of compatible solutes. This effect occurs even for compact states that decelerate the folding as in the case of the intermediate revealed by folding kinetics. [References: 25]
机译：在海藻糖存在下，研究了Cuxa酶的折叠，显示脂肪溶解活性的酶。均衡展开数据表明，海藻糖增加了折叠和展开状态之间的自由能变化。展开动力学揭示了中间体的存在，这是Ca.在溶剂暴露方面折叠60％。海藻糖相对于折叠状态稳定这种中间体。相反，通过折叠动力学揭示的中间体比过渡状态更紧凑，如在字段图中以低变性剂浓度观察到的正斜率，以及可观察速率恒定的降低，以随着海藻糖的增加而折叠专注。该中间体显示器从溶剂（相对于天然状态）埋入的50％面积相比，折叠的过渡状态约为40％，因此似乎脱离折叠通路。海藻糖稳定和盐酸胍破坏了这种紧凑的中间体。展开和折叠动力学都表明，通过海藻糖稳定紧凑的构象状态，同意当前的兼容性溶质的效果模型。即使是用于折叠动力学的中间体的情况下减速折叠的紧凑型状态，也会发生这种效果。 [参考：25]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号