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首页> 外文期刊>Biochemistry >The three-dimensional structure of bovine calcium ion-bound osteocalcin using HNMR spectroscopy
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The three-dimensional structure of bovine calcium ion-bound osteocalcin using HNMR spectroscopy

机译：使用HNMR光谱法的牛钙离子结合骨癌的三维结构

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Structural information on osteocalcin or other noncollagenous bone proteins is very limited. We have solved the three-dimensional structure of calcium bound osteocalcin using H-1 2D NMR techniques and proposed a mechanism for mineral binding. The protons in the 49 amino acid sequence were assigned using standard two-dimensional homonuclear NMR experiments. Distance constraints, dihedral angle constraints, hydrogen bonds, and H-1 and C-13 chemical shifts were all used to calculate a family of 13 structures. The tertiary structure of the protein consisted of an unstructured N terminus and a C-terminal loop (residues 16-49) formed by long-range hydrophobic interactions. Elements of secondary structure within residues 16-49 include type III turns (residues 20-25) and two (x-helical regions (residues 2735 and 41-44). The three Gla residues project from the same face of the helical turns and are surface exposed. The genetic algorithm-molecular dynamics simulation approach was used to place three calcium atoms on the NMR-derived structure. One calcium atom was coordinated by three side chain oxygen atoms, two from Asp30, and one from Gla24. The second calcium atom was coordinated to four oxygen atoms, two from the side chain in Gla 24, and two from the side chain of Gla 21. The third calcium atom was coordinated to two oxygen atoms of the side chain of Gla17. The best correlation of the distances between the uncoordinated Gla oxygen atoms is with the intercalcium distance of 9.43 Angstrom in hydroxyapatite. The structure may provide further insight into the function of osteocalcin. [References: 62]
机译：关于骨钙素或其他非胶原骨蛋白的结构信息非常有限。使用H-1 2D NMR技术解决了钙结合的骨钙蛋白的三维结构，提出了矿物结合的机制。使用标准二维同核NMR实验分配49个氨基酸序列中的质子。距离约束，二偏振角约束，氢键和H-1和C-13化学偏移都是用于计算13种结构的家庭。蛋白质的三级结构由非结构化的N末端和通过远程疏水相互作用形成的C末端环（残基16-49）。残留物16-49内的二级结构的元素包括III型转弯（残基20-25）和两个（X螺旋区（残留物2735和41-44）。从螺旋转弯的同一面上突出三个GLA残留物。表面暴露。使用遗传算法 - 分子动力学模拟方法在NMR衍生的结构上放置三个钙原子。一个钙原子由三个侧链氧原子，来自ASP30的两个，来自GLA24。第二钙原子与四个氧原子配位，两个来自GLA 24中的侧链，以及来自GLA 21的侧链的两个。第三钙原子与GLA17的侧链的两个氧原子配位。距离之间的最佳相关性未配备的Gla氧原子是羟基磷灰石中9.43埃的静脉距离。该结构可以进一步了解骨钙素的功能。[参考文献：62]

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