...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Insights into the Specificity of Thioredoxin Reductase—Thioredoxin Interactions, AStructural and Functional Investigation of the Yeast Thioredoxin Systernt
【24h】

Insights into the Specificity of Thioredoxin Reductase—Thioredoxin Interactions, AStructural and Functional Investigation of the Yeast Thioredoxin Systernt

机译：敏捷探讨毒素还原酶 - 硫杂曲霉素相互作用，酵母患者的结构和功能调查

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The enzymatic activity of thioredoxin reductase enzymes is endowed by at least two redox centers: aflavin and a dithiol/disulfide CXXC motif. The interaction between thioredoxin reductase and thioredoxin isgenerally species-specific, but the molecular aspects related to this phenomenon remain elusive. Here, weinvestigated the yeast cytosolic thioredoxin system, which is composed of NADPH, thioredoxin reductase(ScTrxRl), and thioredoxin 1 (ScTrxl) or thioredoxin 2 (ScTrx2). We showed that ScTrxR I was able to efficientlyreduce yeast thioredoxins (mitochondrial and cytosolic) but failed to reduce the human and Escherichia coltthioredoxin counterparts. To gain insights into this specificity, the crystallographic structure of oxidized ScTrxR Iwas solved at 2.4 A resolution. The protein topology of the redox centers indicated the necessity of a largestructural rearrangement for FAD and thioredoxin reduction using NADPH. Therefore, we modeled a largestructural rotation between the two ScTrxRl domains (based on the previously described crystal structure,PDB code I F6M). Employing diverse approaches including enzymatic assays, site-directed mutagenesis,amino acid sequence alignment, and structure comparisons, insights were obtained about the features involvedin the species-specificity phenomenon, such as complementary electronic parameters between the surfaces ofScTrxRl and yeast thioredoxin enzymes and loops and residues (such as Ser72 in ScTrx2). Finally, structuralcomparisons and amino acid alignments led us to propose a new classification that includes a larger number ofenzymes with thioredoxin reductase activity, neglected in the low/high molecular weight classification.
机译：硫氧嗪还原酶酶的酶活性通过至少两种氧化还原中心提供：aflavin和二硫醇/二硫化物CXXC基序。硫氧嘧啶还原酶与硫氧化嗪的相互作用是特异性物种的特异性，但与这种现象相关的分子方面仍然难以捉摸。在此，我们是由NADPH，硫醚素还原酶（SCTRXR1）和硫氧嗪1（SCTRX1）或硫氧肽2（SCTRX2）组成的酵母细胞溶质硫氧化酶系统。我们展示SCTRXR我能够有效地培训酵母毒素（线粒体和细胞溶质），但未减少人和大肠杆菌的Colthoredoxin对应物。为了进入这种特异性，氧化SCTRXR IWA的晶体结构在2.4分辨率下解决。氧化还原中心的蛋白质拓扑表明使用NADPH的使用和硫辛素的最大值重排的必要性。因此，我们建模了两个SCTRXRL域之间的最大旋转（基于先前描述的晶体结构，PDB代码I F6M）。采用不同方法，包括酶测定，定向诱变，氨基酸序列比对和结构比较，关于该特征所涉及物种特异性现象的洞察力，例如腹腔绿和酵母的表面之间的互补电子参数，以及环残留物（如SCTRX2中的SER72）。最后，结构组成和氨基酸对准LED指导我们提出了一种新的分类，其包括较大数量的具有硫氧嘧啶还原酶活性，在低/高分子量分类中被忽略。

著录项

  • 来源
    《Biochemistry》 |2010年第15期|共10页
  • 作者

    Marcos A. Oliveira; Karen F. Discola; Simone V. Alves; Francisco J. Medrano; Beatriz G. Guimaraes; Luis E. S. Netto;

  • 作者单位

    Department° de Biologia Universidade Estadual Paulista Sao Vicente Brazil;

    Departamento de Genetica e Biologia Evolutiva Institute de Biociencias Universidade de Sao Paulo Sao Paulo Brazil;

    Departamento de Genetica e Biologia Evolutiva Institute de Biociencias Universidade de Sao Paulo Sao Paulo Brazil;

    Departamento de Genetica e Evolucdo lnstituto de Biologia Universidade Estadual de Campinas Campinas;

    Synchrotron Soleil L'Orme de Merisiers Saint Aubin-BP48 Gif-sur-Y vette Cedex France;

    Departamento de Genetica e Biologia Evolutiva Institute de Biociencias Universidade de Sao Paulo Sao Paulo Brazil;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    phenomenon; necessity; molecular;

    机译：现象;必要性;分子;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号