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首页> 外文期刊>Biochemistry >Relationships among Visual Cycle Retinoids, Rhodopsin Phosphorylation, and Phototransduction in Mouse Eyes during Light and Dark Adaptation
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Relationships among Visual Cycle Retinoids, Rhodopsin Phosphorylation, and Phototransduction in Mouse Eyes during Light and Dark Adaptation

机译：在轻质和深色适应期间，视觉循环含量，罗地素磷酸化和鼠标眼中的光电传播的关系

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摘要

Phosphorylation and regeneration of rhodopsin, the prototypical G-protein-coupled receptor, each can influence light and dark adaptation. To evaluate their relative contributions, we quantified rhodopsin, retinoids, phosphorylation, and photosensitivity in mice during a 90 min illumination followed by dark adaptation. During illumination, all-trans-retinyl esters and, to a lesser extent, all-trans-retinal accumulate and reach the steady state in < 1 h. Each major phosphorylation site on rhodopsin reaches a steady state level of phosphorylation at a different time during illumination. The dominant factor that limits dark adaptation is isomerization of retinal. During dark adaptation, dephosphorylation of rhodopsin occurs in two phases. The faster phase corresponds to rapid dephosphorylation of regenerated rhodopsin present at the end of the illumination period. The slower phase corresponds to dephosphorylation of rhodopsin as it forms by regeneration. We conclude that rhodopsin phosphorylation has three physiological functions: it quenches phototransduction, reduces sensitivity during light adaptation, and suppresses bleached rhodopsin activity during dark adaptation.
机译：罗多蛋白酶，原型G蛋白偶联受体的磷酸化和再生，各自可以影响光和暗适应。为了评估其相对贡献，在90分钟照明之后，我们在小鼠中量化了小鼠中的罗多蛋白，含油蛋白，磷酸化和光敏性。在照明期间，全转甲烷基酯和较小程度，全转位式累积并达到<1小时的稳态。在照明期间，roodopsin上的每个主要磷酸化位点达到不同时间的稳定状态磷酸化。限制深色适应的主导因素是视网膜的异构化。在深色适应期间，罗多蛋白酶的去磷酸化发生在两个阶段。更快的相位对应于在照射时段结束时存在的再生紫红素的快速去磷酸化。较慢的相对应于通过再生形式的洛椰菜去磷酸化。我们得出结论，罗地甙磷酸化具有三种生理功能：它淬火光电传递，减少光适应期间的灵敏度，并在深色适应期间抑制漂白的罗经蛋白酶活性。

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  • 来源
    《Biochemistry》 |2010年第11期|共10页
  • 作者

    Kimberly A. Lee; Maria Nawrot; Gregory G. Garwin; John C. Saari; James B. Hurley;

  • 作者单位

    Department of Biochemistry (Box 357350) University of Washington Seattle Washington 98195;

    Department of Ophthalmology (Box 356485) University of Washington Seattle Washington 98195;

    Department of Ophthalmology (Box 356485) University of Washington Seattle Washington 98195;

    Department of Biochemistry (Box 357350) University of Washington Seattle Washington 98195;

    Department of Biochemistry (Box 357350) University of Washington Seattle Washington 98195;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    Relationships; Phosphorylation; Adaptationt;

    机译：关系;磷酸化;适应;

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