Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis

Kishimoto Shinji; Tsunematsu Yuta; Matsushita Takuma; Hara Kodai; Hashimoto Hiroshi; Tang Yi; Watanabe Kenji

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Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis

机译：真菌聚酮化合物生物合成中反式C-甲基转移酶的功能和结构分析

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Biosynthesis of certain fungal polyketide-peptide synthetases involves C-methyltransferase activity that adds one or more S-adenosyl-L-methionine-derived methyl groups to the carbon framework. The previously reported PsoF-MT, the stand-alone C-methyltransferase (MT) from the pseurotin biosynthetic pathway that exists as a domain within a trifunctional didomain enzyme PsoF, was characterized crystallographically and kinetically using mutants with substrate analogs to understand how a transacting C-MT works and compare it to known polyketide synthase-associated C-MTs. This study identified key active-site residues involved in catalysis and substrate recognition, which led us to propose the mechanism of C-methylation and substrate specificity determinants in PsoF-MT.

机译：某些真菌聚酮基肽合成酶的生物合成涉及C-甲基转移酶活性，其将一个或多个S-腺苷-1-甲硫氨酸衍生的甲基衍生的甲基衍生给碳骨架。先前报道的psof-mt，来自伪素生物合成途径的独立的c-甲基转移酶（mt），其作为三官能脱瘤酶Psof的结构域，在晶体上表征，并且使用含有底物类似物的突变体来了解如何交易C. -MT工作并将其与已知的聚酮合成酶相关的C-MT进行比较。该研究确定了催化和基材识别中涉及的关键有效现场残留物，其导致我们提出了在PSOF-MT中的C-甲基化和底物特异性决定簇的机制。

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《Biochemistry》 |2019年第38期|共5页
作者
Kishimoto Shinji; Tsunematsu Yuta; Matsushita Takuma; Hara Kodai; Hashimoto Hiroshi; Tang Yi; Watanabe Kenji;
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Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

Univ Calif Los Angeles Dept Chem &

Biomol Engn Los Angeles CA 90095 USA;

Univ Shizuoka Dept Pharmaceut Sci Shizuoka 4228526 Japan;

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正文语种 eng
中图分类生物化学;
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1. Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis [J] . Kishimoto Shinji, Tsunematsu Yuta, Matsushita Takuma, Biochemistry . 2019,第38期

机译：真菌聚酮化合物生物合成中反式C-甲基转移酶的功能和结构分析
2. Functional and Structural Analyses of the Split-Dehydratase Domain in the Biosynthesis of Macrolactam Polyketide Cremimycin [J] . Kawasaki Daisuke, Miyanaga Akimasa, Chisuga Taichi, Biochemistry . 2019,第48期

机译：甲基聚酮氨基氨基生物合成中分裂脱水酶结构域的功能和结构分析
3. The programming role of trans-acting enoyl reductases during the biosynthesis of highly reduced fungal polyketides [J] . Mary N. Heneghan, Ahmed A. Yakasai, Katherine Williams Chemical science . 2011,第5期

机译：反式烯酰还原酶在高度还原真菌聚酮化合物生物合成过程中的编程作用
4. Determining the specificity of ketosynthase domains from trans-AT polyketide synthases using electrospray ionization-mass spectrometry: functional assignment of biosynthetic pathways. [C] . Matthew Jenner, Sarah Frank, Annette Kampa, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：使用电喷雾电离质谱法测定从反式聚酮化合成酶的酮皂酶结构域的特异性：生物合成途径的功能分配。
5. The Making and Breaking of Lipids: The Characterization of the Protein-Protein and Protein-Substrate Interactions and Bioengineering of Type II Fatty Acid Synthases FabA and AcpP and the Structural and Functional Characterization of Iterative Type I Trans-Acting Enoyl-Reductase, LovC Polyketide Synthase. [D] . Nguyen, Chi Hanh Thi. 2014

机译：脂质的产生和断裂：II型脂肪酸合酶FabA和AcpP的蛋白质-蛋白质和蛋白质-底物相互作用的表征和生物工程以及I型迭代反式烯丙基还原酶，LovC聚酮合酶的结构和功能表征。
6. Functional and structural analysis of programmed C-methylation in the biosynthesis of the fungal polyketide citrinin [O] . Philip A. Storm, Dominik A. Herbst, Timm Maier, -1

机译：程序化的C-甲基化在真菌性聚酮化合物citrinin生物合成中的功能和结构分析
7. Functional and Structural Analysis of Programmed C-Methylation in the Biosynthesis of the Fungal Polyketide Citrinin [O] . Storm, Philip A., Herbst, Dominik A., Maier, Timm, 2017

机译：真菌聚酮化合物桔霉素生物合成中程序化C-甲基化的功能和结构分析

Functional and Structural Analyses of trans C-Methyltransferase in Fungal Polyketide Biosynthesis

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