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Spatially Resolved Membrane Transport in a Single Cell Imaged by Second Harmonic Light Scattering

机译:通过二次谐波光散射成像的单个细胞中的空间分离的膜运输

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摘要

We demonstrate that time-resolved second harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions, which appear to be enduring higher stress, exhibit slower transport rates. It is proposed that this stress-transport relation may be a result of local enrichment of membrane rigidifiers as part of a response to maintain membrane integrity under strain.
机译:我们证明时分分辨的第二次谐波(SH)光散射,当施加为成像模型时,可用于在空间地解析在活细胞中延伸穿过膜的分子的吸附和运输速率。 作为代表性的例子,我们测量含有人类皮肤成纤维细胞中的血浆膜的两亲子离子,孔雀石绿的被动传输。 分辨率的SH图像分析显示,膜区域似乎持久的压力,表现出较慢的运输速率。 提出,这种应力传输关系可能是膜刚性纤维件的局部富集的结果,作为保持菌株下膜完整性的反应的一部分。

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  • 来源
    《Biochemistry》 |2019年第14期|共4页
  • 作者单位

    Temple Univ Dept Chem 1901 N 13th St Philadelphia PA 19122 USA;

    Temple Univ Dept Chem 1901 N 13th St Philadelphia PA 19122 USA;

    Delaware State Univ Opt Sci Ctr Appl Res Dover DE 19904 USA;

    Temple Univ Dept Chem 1901 N 13th St Philadelphia PA 19122 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

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