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首页> 外文期刊>Biochemistry >Two ABC Transporters and a Periplasmic Metallochaperone Participate in Zinc Acquisition in Paracoccus denitrificans
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Two ABC Transporters and a Periplasmic Metallochaperone Participate in Zinc Acquisition in Paracoccus denitrificans

机译：两个ABC转运蛋白和周质金属体Chaperone参与在帕拉科克脱氮网中的锌获取

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摘要

Bacteria must acquire the essential element zinc from extremely limited environments, and this function is performed largely by ATP binding cassette (ABC) transporters. These systems rely on a periplasmic or extracellular solute binding protein (SBP) to bind zinc specifically with a high affinity and deliver it to the membrane permease for import into the cytoplasm. However, zinc acquisition systems in bacteria may be more complex, involving multiple transporters and other periplasmic or extracellular zinc binding proteins. Here we describe the zinc acquisition functions of two zinc SBPs (ZnuA and AztC) and a novel periplasmic metallochaperone (AztD) in Paracoccus denitrificans . ZnuA was characterized in vitro and demonstrated to bind as many as 5 zinc ions with a high affinity. It does not interact with AztD, in contrast to what has been demonstrated for AztC, which is able to acquire a single zinc ion through associative transfer from AztD. Deletions of the corresponding genes singly and in combination show that either AztC or ZnuA is sufficient and essential for robust growth in zinc-limited media. Although AztD cannot support transport of zinc into the cytoplasm, it likely functions to store zinc in the periplasm for transfer through the AztABCD system.
机译：细菌必须从极限的环境中获取基本元素锌，并且该功能主要由ATP结合盒（ABC）转运蛋白进行。这些系统依赖于周质或细胞外溶质结合蛋白（SBP），以具有高亲和力的特异性结合锌，并将其递送至膜允许进入细胞质的透气释放。然而，细菌中的锌获取系统可能更复杂，涉及多个转运蛋白和其他周质或细胞外锌结合蛋白。在这里，我们描述了两个锌Sbps（Znua和Aztc）的锌获取功能和在裂微裂微裂变物中的新型周质Metallocherone（AZTD）。 Znua在体外表征，并证明了具有高亲和力的多达5个锌离子。它与AZTD不相互作用，与AZTC的证明相反，能够通过来自AZTD的关联转移来获取单个锌离子。单独缺失相应的基因并组合表明，AZTC或Znua是足够的，对于锌有限介质的鲁棒增长是必不可少的。尽管AZTD不能支持锌的传输到细胞质中，但它可能有效地将锌存放在周子中的锌以通过AZTABCD系统转移。

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  • 来源
    《Biochemistry》 |2019年第2期|共11页
  • 作者

    DurgaP. Neupane; Santosh Kumar; Erik T. Yukl;

  • 作者单位

    Department of Chemistry and Biochemistry New Mexico State University;

    Department of Biological Sciences University of Texas at Dallas;

    Department of Chemistry and Biochemistry New Mexico State University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
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