Precursor–Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation

Marta Wojnowska; Joseph Gault; Shee Chien Yong; Carol V. Robinson; Ben C. Berks

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Precursor–Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation

机译：用新的受体复合物制剂探测双精氨酸蛋白转运途径中的前体受体相互作用

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The twin arginine translocation (Tat) system moves folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plant chloroplasts. Signal peptide-bearing substrates of the Tat pathway (precursor proteins) are recognized at the membrane by the TatBC receptor complex. The only established preparation of the TatBC complex uses the detergent digitonin, rendering it unsuitable for biophysical analysis. Here we show that the detergent glyco-diosgenin (GDN) can be used in place of digitonin to isolate homogeneous TatBC complexes that bind precursor proteins with physiological specificity. We use this new preparation to quantitatively characterize TatBC–precursor interactions in a fully defined system. Additionally, we show that the GDN-solubilized TatBC complex co-purifies with substantial quantities of phospholipids.

机译：双精氨酸易位（TAT）系统在细菌的细胞质膜上移动折叠的蛋白质和植物叶绿体的紫胶膜。通过TATBC受体复合物在膜上在膜上识别出TAT路径（前体蛋白）的信号肽亚壳。唯一建立的TATBC复合物的制备使用洗涤剂Digitonin，使其不适合生物物理学分析。在这里，我们表明可以使用洗涤剂Glyco-Diosgenin（GDN）代替Digitonin以分离与生理特异性结合前体蛋白质的均匀TATBC复合物。我们使用这种新的准备来定量表征完全定义的系统中的TATBC-PreceoroR交互。另外，我们表明GDN溶解的TATBC复合物与大量的磷脂共同纯化。

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《Biochemistry》 |2018年第10期|共9页
作者
Marta Wojnowska; Joseph Gault; Shee Chien Yong; Carol V. Robinson; Ben C. Berks;
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作者单位

Department of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU United Kingdom;

Physical and Theoretical Chemistry Laboratory Department of Chemistry University of Oxford South Parks Road Oxford OX1 3QZ United Kingdom;

Department of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU United Kingdom;

Physical and Theoretical Chemistry Laboratory Department of Chemistry University of Oxford South Parks Road Oxford OX1 3QZ United Kingdom;

Department of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU United Kingdom;

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正文语种 eng
中图分类生物化学;
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1. Precursor–Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation [J] . Marta Wojnowska, Joseph Gault, Shee Chien Yong, Biochemistry . 2018,第10期

机译：用新的受体复合物制剂探测双精氨酸蛋白转运途径中的前体受体相互作用
2. The early mature part of bacterial twin-arginine translocation (Tat) precursor proteins contributes to TatBC receptor binding [J] . Agnes Ulfig, Roland Freudl The Journal of biological chemistry . 2018,第19期

机译：细菌双精氨酸易位（Tat）前体蛋白的早期成熟部分有助于TatBC受体结合
3. The early mature part of bacterial twin-arginine translocation (Tat) precursor proteins contributes to TatBC receptor binding [J] . Agnes Ulfig, Roland Freudl The Journal of biological chemistry . 2018,第19期

机译：细菌双精氨酸易位（Tat）前体蛋白的早期成熟部分有助于TatBC受体结合
4. Using MALDI-TOF-MS to probe protein-ligand interactions of G-protein coupled receptors incorporated into stable polymerized planar supported lipid bilayers [C] . Erin M. Johnson, James R. Joubert, S. Scott Saavedra, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：使用MALDI-TOF-MS探测掺入稳定聚合平面的G-蛋白偶联受体的蛋白质 - 配体相互作用的脂质双层
5. Examining the Effect of Protein Arginine Methylation on G Protein-Coupled Receptor Signaling and Protein-Protein Interactions with Modulators of D2 Signaling [D] . Bowitch, Alexander. 2021

机译：检查蛋白质精氨酸甲基化对G蛋白偶联受体信号传导和蛋白质 - 蛋白质 - 蛋白质 - 蛋白质 - 与D2信号调节剂的影响
6. Precursor–Receptor Interactions in the TwinArginine Protein Transport Pathway Probed with a New Receptor ComplexPreparation [O] . Marta Wojnowska, §, Joseph Gault, -1

机译：双胞胎中的前体-受体相互作用用新的受体复合物探测精氨酸蛋白的运输途径制备
7. The early mature part of bacterial twin-arginine translocation (Tat) precursor proteins contributes to TatBC receptor binding [O] . Agnes Ulfig, Roland Freudl 2018

机译：细菌双精氨酸易位（TAT）前体蛋白的早期成熟部分有助于TATBC受体结合
8. Studies of the Mu-Opioid Receptor/G-protein Complex Affinity Co-Purified and Membrane Preparations from 7315c Cells. [R] . Gosse, M. E. 1989

机译：来自7315c细胞的mu-阿片受体/ G蛋白复合物亲和共纯化和膜制剂的研究。

Precursor–Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation

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