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首页> 外文期刊>Biochemistry >Comobility of GABARAP and Phosphatidylinositol 4-Kinase 2A on Cytoplasmic Vesicles
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Comobility of GABARAP and Phosphatidylinositol 4-Kinase 2A on Cytoplasmic Vesicles

机译：糖皮皮和磷脂酰肌醇4-激酶2a在细胞质囊泡中的可中性

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摘要

We previously reported that recruitment of the type IIA phosphatidylinositol 4-kinase (PI4K2A) to autophagosomes by GABARAP, a member of the Atg8 family of autophagy-related proteins, is important for autophagosome-lysosome fusion. Because both PI4K2A and GABARAP have also been implicated in the intracellular trafficking of plasma membrane receptors in the secretory/endocytic pathway, we characterized their interaction in cells under nonautophagic conditions. Fluorescence fluctuation spectroscopy measurements revealed that GABARAP exists predominantly as a cytosolic monomer in flve cells, but is recruited to small cytoplasmic vesicles upon overexpression of PI4K2A..C-Terminal lipidation of GABARAP, which is essential for its autophagic activities, is not necessary for its recruitment to these PI4K2A-containing transport vesicles .However, a GABARAP truncation mutant lacking C-terminal residues 103-117 fails to bind to PI4K2A, is not recruited to cytoplasmic vesicles, and does not codistribute with PI4K2A on subcellular organelles. These observations suggest that the PI4K2A-GABARAP interaction plays a role in membrane trafficking both under autophagic and nonautophagic conditions.
机译：我们以前报道，通过加巴帕募集IIA型磷脂酰肌醇4-激酶（PI4K2A）对自噬相关蛋白的ATG8系列的成员，对自噬体 - 溶酶体融合是重要的。因为PI4K2A和Gabarap两者也涉及分泌物/内吞的途径中的质膜受体的细胞内运输，所以我们在非自杀式条件下表征它们在细胞中的相互作用。荧光波动光谱测量显示，加布帕皮主要存在于发病细胞中的细胞溶质单体，但在过表达对Pi4K2A的过表达时募集到小型细胞质囊泡。对于其自噬活性至关重要，对其自噬作用至关重要，这是不是必需的募集到这些含PI4K2A的运输囊泡。然而，缺乏C末端残基103-117的加布拉帕截短突变体未能结合PI4K2A，未募集到细胞质囊泡，并且不与亚细胞有机体上的PI4K2a致译合。这些观察结果表明，PI4K2A-GABARAP相互作用在自噬和非效应条件下在膜运输中发挥作用。

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  • 来源
    《Biochemistry》 |2018年第26期|共4页
  • 作者

    Chen Yan; Sun Hui-Qiao; Eichorst John P.; Albanesi Joseph P.; Yin Helen; Mueller Joachim D.;

  • 作者单位

    Univ Minnesota Sch Phys &

    Astron Minneapolis MN 55455 USA;

    Univ Texas Southwestern Med Ctr Dallas Dept Physiol Dallas TX 75390 USA;

    Univ Minnesota Sch Phys &

    Astron Minneapolis MN 55455 USA;

    Univ Texas Southwestern Med Ctr Dallas Dept Pharmacol Dallas TX 75390 USA;

    Univ Texas Southwestern Med Ctr Dallas Dept Physiol Dallas TX 75390 USA;

    Univ Minnesota Sch Phys &

    Astron Minneapolis MN 55455 USA;

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  • 正文语种 eng
  • 中图分类 生物化学;
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