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Developing a Fluorescent Toolbox To Shed Light on the Mysteries of RNA

机译：开发一个荧光工具箱在RNA的谜团上脱光

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src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2017/bichaw.2017.56.issue-39/acs.biochem.7b00510/20170927/images/medium/bi-2017-00510p_0005.gif">Technologies that detect and image RNA have illuminated the complex roles played by RNA, redefining the traditional and superficial role first outlined by the central dogma of biology. Because there is such a wide diversity of RNA structure arising from an assortment of functions within biology, a toolbox of approaches have emerged for investigation of this important class of biomolecules. These methods are necessary to detect and elucidate the localization and dynamics of specific RNAs and in doing so unlock our understanding of how RNA dysregulation leads to disease. Current methods for detecting and imaging RNA include in situ hybridization techniques, fluorescent aptamers, RNA binding proteins fused to fluorescent reporters, and covalent labeling strategies. Because of the inherent diversity of these methods, each approach comes with a set of strengths and limitations that leave room for future improvement. This perspective seeks to highlight the most recent advances and remaining challenges for the wide-ranging toolbox of technologies that illuminate RNA’s contribution to cellular complexity.
机译：src =“http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2017/bichaw.2017.56.issue-39/acs.biochem.7b00510/20170927/images/medium /bi-2017-00510p_0005.gif“检测和图像RNA的”技术RNA照亮了RNA发挥的复杂角色，重新定义了生物学中央教条中首先概述的传统和肤浅的作用。因为存在从生物学中的各种作用产生的RNA结构具有如此广泛的RNA结构，因此出现了一种方法的工具箱，用于调查这类重要的生物分子。这些方法是检测和阐明特定RNA的本地化和动态的方法，并在此操作中解锁我们对RNA失呼量如何导致疾病的理解。用于检测和成像RNA的目前的方法包括原位杂交技术，荧光体适体，RNA结合蛋白融合给荧光报告，以及共价标记策略。由于这些方法的固有多样性，每种方法都有一系列的优势和限制，使未来的改善空间。这种观点旨在突出最新的进展和宽敞的工具框架的挑战，可以照亮RNA对细胞复杂性的贡献的贡献。

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  • 来源
    《Biochemistry》 |2017年第39期|共9页
  • 作者

    Seth C. Alexander; Neal K. Devaraj;

  • 作者单位

    Department of Chemistry and Biochemistry University of California San Diego La Jolla California 92093 United States;

    Department of Chemistry and Biochemistry University of California San Diego La Jolla California 92093 United States;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
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