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Activation of Proton Translocation by Respiratory Complex I

机译：呼吸络合物I的质子易位激活

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摘要

Activation of proton pumping by reconstituted and native membrane-bound Complex I was studied using optical electric potential- and pH-sensitive probes. We find that reconstituted Complex I has a delay in proton translocation, which is significantly longer than the delay in quinone reductase activity, indicating an initially decoupled state of Complex I. Studies of the amount of NADH required for the activation of pumping indicate the prerequisite of multiple turnovers. Proton pumping by Complex I was also activated by NADPH, excluding significant reduction of Complex I and a preexisting Delta psi as activation factors. Co-reconstitution of Complex I and ATPase did not indicate an increased membrane permeability for protons in the uncoupled Complex I state. The delay in Complex I proton pumping activation was also observed in subbacterial vesicles. While it is negligible at room temperature, it strongly increases at a lower temperature. We conclude that Complex I undergoes a conversion from a decoupled state to a coupled state upon activation. The possible origins and importance of the observed phenomenon are discussed.
机译：使用光学电位和pH敏感探针研究了由重构和天然膜结合络合物I的质子泵送的激活。我们发现重组复合物I具有质子易位的延迟，这显着长于醌还原酶活性的延迟，表明最初解耦的复合物状态。研究泵送激活所需的NADH量的研究表明了所需的前提多次失误。由络合物泵送的质子泵送I也由NADPH激活，除了显着减少复杂的I和预先存在的Delta PSI作为激活因子。复合体和ATP酶的共同重构并未表明在未耦合的络合物I状态下对质子的膜渗透性增加。在近细菌囊泡中也观察到复合物I质子泵送活化的延迟。虽然室温可忽略不计，但它在较低温度下强烈增加。我们得出结论，在激活时，复杂的I经历从分离状态转换到耦合状态。讨论了观察到的现象的可能起源和重要性。

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  • 来源
    《Biochemistry》 |2017年第42期|共7页
  • 作者

    Belevich Nikolai; von Ballmoos Christoph; Verkhovskaya Marina;

  • 作者单位

    Univ Helsinki Inst Biotechnol POB 65 Viikinkaari 1 FIN-00014 Helsinki Finland;

    Univ Bern Dept Chem &

    Biochem Freiestr 3 CH-3012 Bern Switzerland;

    Univ Helsinki Inst Biotechnol POB 65 Viikinkaari 1 FIN-00014 Helsinki Finland;

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  • 正文语种 eng
  • 中图分类 生物化学;
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