ECFP, the first usable '/> Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins
【24h】

Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins

机译：发色团异构体稳定化对于青色荧光蛋白的有效荧光至关重要

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

src="http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2017/bichaw.2017.56.issue-49/acs.biochem.7b01088/20171206/images/medium/bi-2017-01088f_0006.gif">ECFP, the first usable cyan fluorescent protein (CFP), was obtained by adapting the tyrosine-based chromophore environment in green fluorescent protein to that of a tryptophan-based one. This first-generation CFP was superseded by the popular Cerulean, CyPet, and SCFP3A that were engineered by rational and random mutagenesis, yet the latter CFPs still exhibit suboptimal properties of pH sensitivity and reversible photobleaching behavior. These flaws were serendipitously corrected in the third-generation CFP mTurquoise and its successors without an obvious rationale. We show here that the evolution process had unexpectedly remodeled the chromophore environment in second-generation CFPs so they would accommodate a different isomer, whose formation is favored by acidic pH or light irradiation and which emits fluorescence much less efficiently. Our results illustrate how fluorescent protein engineering based solely on fluorescence efficiency optimization may affect other photophysical or physicochemical parameters and provide novel insights into the rational evolution of fluorescent proteins with a tryptophan-based chromophore.
机译：src =“http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2017/bichaw.2017.56.issue-49/acs.biochem.7b01088/20171206/images/medium /bi-2017-01088f_0006.gif"2cfp，首先可用的青色荧光蛋白（CFP）是通过在绿色荧光蛋白中与色氨酸的基于色氨酸蛋白质中的一种的基于色团荧光蛋白（CFP）获得。该第一代CFP被理性和随机诱变设计的流行的Cerulan，Cypet和SCFP3A取代，但后者CFP仍然表现出pH敏感性和可逆光漂移行为的次优。在第三代CFP Mturquoise及其继任者中，这些缺陷无明显纠正。我们在这里展示了进化过程意外地在第二代CFP中重塑了发色团环境，因此它们将容纳不同的异构体，其形成由酸性pH或光照射而受到青睐，并且其效率低得多地发出荧光。我们的结果说明了荧光蛋白质工程如何完全基于荧光效率优化，可能影响其他光物理或物理化学参数，并为荧光蛋白的合理演变提供了一种具有色氨酸的发色团的合理演变。

著录项

  • 来源
    《Biochemistry》 |2017年第49期|共5页
  • 作者

    Guillaume Gotthard; David von Stetten; Damien Clavel; Marjolaine Noirclerc-Savoye; Antoine Royant;

  • 作者单位

    European Synchrotron Radiation Facility F-38043 Grenoble France;

    European Synchrotron Radiation Facility F-38043 Grenoble France;

    Univ. Grenoble Alpes CNRS CEA IBS (Institut de Biologie Structurale) F-38000 Grenoble France;

    Univ. Grenoble Alpes CNRS CEA IBS (Institut de Biologie Structurale) F-38000 Grenoble France;

    European Synchrotron Radiation Facility F-38043 Grenoble France;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号