The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

YamanoS.; HakuK.; YamanakaT.; DaiJ.; TakayamaT.; ShoharaR.; TachiK.; IshiokaM.; HanataniS.; KarunagaranS.; WadaK.; MoursiA.M.

首页> 外文期刊>Biomaterials >The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

【24h】

The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

机译：生物活性胶原膜释放PDGF或GDF-5对骨再生的影响

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Regenerative procedures using barrier membrane technology are presently well established in periodontal/endodontic surgery. The objective of this study was to compare the subsequent effects of the released platelet-derived growth factor (PDGF) and growth/differentiation factor 5 (GDF-5) from collagen membranes (CMs) on bone regeneration invitro and invivo. Invitro studies were conducted using MC3T3-E1 mouse preosteoblasts cultured with or without factors. Cell viability, cell proliferation, alkaline phosphatase (ALP) activity and bone marker gene expression were then measured. Invivo studies were conducted by placing CMs with low or high dose PDGF or GDF-5 in rat mandibular defects. At 4 weeks after surgery new bone formation was measured using μCT and histological analysis. The results of invitro studies showed that CM/GDF-5 significantly increased ALP and cell proliferation activities without cytotoxicity in MC3T3-E1 cells when compared to CM/PDGF or CM alone. Gene expression analysis revealed that Runx2 and Osteocalcin were significantly increased in CM/GDF-5 compared to CM/PDGF or control. Quantitative and qualitative μCT and histological analysis for new bone formation revealed that although CM/PDGF significantly enhanced bone regeneration compared to CM alone or control, CM/GDF-5 significantly accelerated bone regeneration to an even greater extent than CM/PDGF. The results also showed that GDF-5 induced new bone formation in a dose-dependent manner. These results suggest that this strategy, using a CM carrying GDF-5, might lead to an improvement in the current clinical treatment of bone defects for periodontal and implant therapy.

机译：目前，在牙周/胸腺外科中，目前使用阻隔膜技术的再生程序。本研究的目的是将释放的血小板衍生的生长因子（PDGF）和生长/分化因子5（GDF-5）与胶原膜（CMS）进行的随后的效果比较骨再生invitro和Invivo。使用与或没有因素培养的MC3T3-E1小鼠预牙细胞进行invitro的研究。然后测量细胞活力，细胞增殖，碱性磷酸酶（ALP）活性和骨标记基因表达。通过在大鼠下颌缺陷中将CMS放置CMS来进行Invivo研究，以大鼠下颌缺损将CMS放置。在手术后4周，使用μCT和组织学分析测量新的骨形成。 invitro的研究结果表明，与cm / pdgf或cm相比，cm / gdf-5在MC3T3-E1细胞中没有细胞毒性而显着增加ALP和细胞增殖活性。基因表达分析表明，与CM / PDGF或对照相比，润荷X2和骨钙蛋白显着增加Cm / GdF-5。用于新骨形成的定量和定性μCT和组织学分析，虽然CM / PDGF与单独或对照相比，CM / PDGF显着增强了骨再生，CM / GDF-5显着加速了骨再生至比Cm / PDGF更大的程度。结果还表明GDF-5以剂量依赖性方式诱导新的骨形成。这些结果表明，这种策略使用CM携带GDF-5，可能导致牙周和植入治疗的目前临床治疗的改善。

著录项

来源
《Biomaterials》 |2014年第8期|共8页
作者
YamanoS.; HakuK.; YamanakaT.; DaiJ.; TakayamaT.; ShoharaR.; TachiK.; IshiokaM.; HanataniS.; KarunagaranS.; WadaK.; MoursiA.M.;
展开▼
作者单位

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics New York University College of Dentistry New York NY 10010 United;

Department of Prosthodontics The University of Tennessee College of Dentistry Memphis TN 38103;

Department of Periodontics University of Pennsylvania School of Dental Medicine Philadelphia PA;

Department of Pediatric Dentistry New York University College of Dentistry New York NY 10010;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物医学工程;
关键词
Bone regeneration; Collagen membrane; GDF-5; Growth factor; PDGF;

机译：骨再生;胶原膜;GDF-5;生长因子;PDGF;

相似文献

外文文献
中文文献
专利

1. The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration [J] . YamanoS., HakuK., YamanakaT., Biomaterials . 2014,第8期

机译：生物活性胶原膜释放PDGF或GDF-5对骨再生的影响
2. Comparison of the Physical Properties and In vivo Bioactivities of Silkworm-Cocoon-Derived Silk Membrane, Collagen Membrane, and Polytetrafluoroethylene Membrane for Guided Bone Regeneration [J] . Yong-Yun Ha, Young-Wook Park, HaeYong Kweon Macromolecular Research . 2014,第9期

机译：桑蚕茧丝膜，胶原膜和聚四氟乙烯膜用于引导骨再生的物理性质和体内生物活性的比较
3. Efficacy of using PDGF and xenograft with or without collagen membrane for bone regeneration around immediate implants with induced dehiscence-type defects: A microcomputed tomographic study in dogs [J] . Journal of Periodontology . 2013,第3期

机译：在有诱发裂开型缺损的即刻植入物周围使用PDGF和异种移植物（有或没有胶原膜）进行骨再生的功效：狗的微计算机断层扫描研究
4. CONTROLLED RELEASE OF PDGF-BB FROM TRICALCIUM PHOSPHATE (TCP)/CHITOSAN MICROGRANULES FOR ENHANCED BONE REGENERATION [C] . Jue Yeon Lee, Jeong Hoon Rhyu, Sang Mun Han, Proceedings of the 29th Annual Meeting of the Controlled Release Society . 2002

机译：控制性释放磷酸三钙（TCP）/壳聚糖微粒的PDGF-BB以增强骨骼再生
5. Localized release of platelet-derived growth factor (PDGF) in the development of a collagen based tissue repair and regeneration model [D] . Tsao, Christopher 2013

机译：基于胶原的组织修复和再生模型发展过程中血小板衍生生长因子（PDGF）的局部释放
6. The Release of the Bromodomain Ligand NN-Dimethylacetamide Adds Bioactivity to a Resorbable Guided Bone Regeneration Membrane in a Rabbit Calvarial Defect Model [O] . Barbara Siegenthaler, Chafik Ghayor, Nisarat Ruangsawasdi, 2020

机译：Bromodomain配体NN-二甲基乙酰胺的释放为兔颅盖缺损模型中的可吸收导向骨再生膜增加了生物活性。
7. The Release of the Bromodomain Ligand N,N-Dimethylacetamide Adds Bioactivity to a Resorbable Guided Bone Regeneration Membrane in a Rabbit Calvarial Defect Model [O] . Barbara Siegenthaler, Chafik Ghayor, Nisarat Ruangsawasdi, 2020

机译：Bromodomain配体N，N-二甲基乙酰胺的释放增加了兔颅骨缺陷模型中可再吸收的引导骨再生膜的生物活性
8. Guided Bone Regeneration in Long-Bone Defects with a Structural Hydroxyapatite Graft and Collagen Membrane. [R] . Guda, T., Walker, J. A., Singleton, B. M., 2013

机译：结构羟基磷灰石移植物和胶原膜引导骨缺损的长骨缺损。

The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

摘要

著录项

相似文献

相关主题

期刊订阅