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首页> 外文期刊>Biomaterials >The effect of mechanical extension stimulation combined with epithelial cell sorting on outcomes of implanted tissue-engineered muscular urethras
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The effect of mechanical extension stimulation combined with epithelial cell sorting on outcomes of implanted tissue-engineered muscular urethras

机译：机械延伸刺激结合上皮细胞分类对植入组织工程肌肉尿道的结果的影响

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摘要

Urethral defects are common and frequent disorders and are difficult to treat. Simple natural or synthetic materials do not provide a satisfactory curative solution for long urethral defects, and urethroplasty with large areas of autologous tissues is limited and might interfere with wound healing. In this study, adipose-derived stem cells were used. These cells can be derived from a wide range of sources, have extensive expansion capability, and were combined with oral mucosal epithelial cells to solve the problem of finding seeding cell sources for producing the tissue-engineered urethras. We also used the synthetic biodegradable polymer poly-glycolic acid (PGA) as a scaffold material to overcome issues such as potential pathogen infections derived from natural materials (such as de-vascular stents or animal-derived collagen) and differing diameters. Furthermore, we used a bioreactor to construct a tissue-engineered epithelial-muscular lumen with a double-layer structure (the epithelial lining and the muscle layer). Through these steps, we used an epithelial-muscular lumen built invitro to repair defects in a canine urethral defect model (1cm). Canine urethral reconstruction was successfully achieved based on image analysis and histological techniques at different time points. This study provides a basis for the clinical application of tissue engineering of an epithelial-muscular lumen.
机译：尿道缺陷是常见和频繁的疾病，难以治疗。简单的天然或合成材料不能为长尿道缺陷提供令人满意的疗效，并且具有大面积的自体组织的尿道术有限，可能会干扰伤口愈合。在该研究中，使用脂肪衍生的干细胞。这些细胞可以源自广泛的来源，具有广泛的膨胀能力，并与口腔粘膜上皮细胞组合以解决寻找制备组织工程尿道的种子细胞来源的问题。我们还使用合成的可生物降解的聚合物聚乙醇酸（PGA）作为支架材料，以克服诸如衍生自天然材料（例如去血管支架或动物衍生的胶原）和不同直径的潜在病原体感染的问题。此外，我们使用生物反应器来构建具有双层结构（上皮衬里和肌肉层）的组织工程的上皮肌腔。通过这些步骤，我们使用上皮肌肉腔内invitro来修复犬尿道缺陷模型（1cm）的缺陷。基于不同时间点的图像分析和组织学技术成功地实现了犬尿道重建。本研究为上皮肌法腔组织工程提供了临床应用的基础。

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  • 来源
    《Biomaterials》 |2014年第1期|共8页
  • 作者

    FuQ.; DengC.-L.; ZhaoR.-Y.; WangY.; CaoY.;

  • 作者单位

    Department of Urology Shanghai 6th People's Hospital Shanghai Jiao Tong University Shanghai;

    Department of Plastic and Reconstructive Surgery Shanghai 6th People's Hospital Shanghai Jiao;

    Department of Urology Shanghai 6th People's Hospital Shanghai Jiao Tong University Shanghai;

    Department of Urology Shanghai 6th People's Hospital Shanghai Jiao Tong University Shanghai;

    Department of Plastic and Reconstructive Surgery Shanghai 9th People's Hospital Shanghai Jiao;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
  • 关键词

    Adipose-derived stem cells; Bioreactor; Oral mucosal epithelial cell; Tissue engineering; Urethral defect;

    机译：脂肪衍生的干细胞;生物反应器;口腔粘膜上皮细胞;组织工程;尿道缺陷;

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