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Association of a single nucleotide polymorphism in ribosomal protein L27a gene with marbling in Japanese Black beef cattle

机译:核糖体蛋白L27a基因单核苷酸多态性与日本黑肉牛大理石花纹的关联

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摘要

Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The c2-11#2 expressed sequence tag (EST) has been previously shown to possess expression difference in musculus longissimus muscle between low-marbled and high-marbled steer groups, and to be located within genomic region of a quantitative trait locus for marbling. Thus, the ribosomal protein L27a (RPL27A) gene containing the c2-11#2 EST sequence was considered as a positional candidate for the gene responsible for marbling. In the present study, a single nucleotide polymorphism (SNP) in the promoter region of the RPL27A, referred to as g.3109537C > T, was detected between the 2 steer groups. The SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. The effect of genotypes of the SNP on the predicted breeding value for subcutaneous fat thickness was not statistically significant. These findings suggest that the RPL27A SNP may be useful for effective marker-assisted selection to increase the levels of marbling in Japanese Black beef cattle.
机译:根据肌内脂肪的数量和分布来定义大理石花纹是日本肉牛的一项重要经济特征。先前已证明c2-11#2表达的序列标签(EST)在低大理石和高大理石-牛皮组之间的长肌小肌肉中具有表达差异,并且位于大理石的数量性状基因座的基因组区域内。因此,包含c2-11#2 EST序列的核糖体蛋白L27a(RPL27A)基因被认为是负责大理石花纹的基因的候选位置。在本研究中,在两个转向组之间检测到RPL27A启动子区域的单核苷酸多态性(SNP),称为g.3109537C>T。通过使用日本黑肉牛种群的分析,SNP与牛肉大理石纹标准号的预期育种值相关。 SNP基因型对皮下脂肪厚度的预期繁殖值的影响在统计学上不显着。这些发现表明,RPL27A SNP可用于有效的标记辅助选择,以增加日本黑肉牛的大理石花纹水平。

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