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首页> 外文期刊>Animal Science Journal >Application of time-resolved fluorometry to immunoassays for bovine reproductive hormones.
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Application of time-resolved fluorometry to immunoassays for bovine reproductive hormones.

机译:时间分辨荧光法在牛生殖激素免疫测定中的应用。

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摘要

The principle of time-resolved fluorometry with lanthanide chelates was established in the 1980s, but in the field of animal sciences it has not been widely applied to immunoassays. However, immunoassays that utilize time-resolved fluorometry are possible alternatives to radioimmunoassays, since they can attain high sensitivity without safety risks. In this short review, we introduce the development of time-resolved immunoassays for inhibin A, inhibin B and follicle-stimulating hormone (FSH), and describe their application to the investigation of FSH regulation in male and female cattle. The results obtained using these newly developed immunoassays indicate that inhibin A acts as a feedback regulator for FSH secretion in female cattle, whereas inhibin A, and probably inhibin B, do so in male cattle..
机译:用镧系元素螯合剂进行时间分辨荧光分析的原理是在1980年代确立的,但在动物科学领域,它尚未广泛应用于免疫分析。但是,利用时间分辨荧光法的免疫测定法可能是放射免疫测定法的替代方法,因为它们可以实现高灵敏度而无安全隐患。在这篇简短的评论中,我们介绍了抑制素A,抑制素B和促卵泡激素(FSH)的时间分辨免疫分析方法的发展,并描述了它们在雄性和雌性牛FSH调节研究中的应用。使用这些新近开发的免疫测定方法获得的结果表明,抑制素A充当雌性牛FSH分泌的反馈调节剂,而抑制素A以及可能抑制素B在雄性牛中发挥作用。

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