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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Production of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization
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Production of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization

机译：使用Rhizopus Oryzae的固态发酵生产植酸酶：新型菌株改进方法和纯化和表征研究

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Present study introduces linseed oil cake as a novel substrate for phytase production by Rhizopus oryzae. Statistical approach was employed to optimize various medium components under solid state fermentation (SSF). An overall 8.41-fold increase in phytase production was achieved at the optimum concentrations (w/w, mannitol, 2.05%; ammonium sulfate, 2.84% and phosphate, 0.38%). Further enhancement by 59% was observed due to a novel strain improvement approach. Purified phytase (～34kDa) showed optimal temperature of 45°C, dual pH optima at 1.5 and 5.5 and possesses high catalytic efficiency (2.38×10~6M~(-1)s~(-1)). Characterization study demonstrates the phytase as highly thermostable and resistant to proteolysis, heavy metal ions, etc. Furthermore, an improved HPLC method was introduced to confirm the ability of phytase to degrade phytic acid completely and was found to be an efficient method.
机译：目前研究将亚麻籽油蛋糕作为Rhizopus Oryzae的植酸酶产生的新型衬底。 使用统计方法在固态发酵（SSF）下优化各种培养基组分。 以最佳浓度（W / W，甘露醇，2.05％;硫酸铵，2.84％和磷酸盐，0.38％）实现了植酸酶产生的总体上8.41倍倍增的增加。 由于新的菌株改善方法，观察到进一步提高59％。 纯化的植酸酶（〜34kDA）显示出最佳温度为45℃，双pH值为1.5和5.5，具有高催化效率（2.38×10〜6M〜（-1）S〜（-1））。 表征研究证明了植物酶作为高温稳定性和抗蛋白水解，重金属离子等的抗性。此外，还引入了一种改进的HPLC方法以确认植酸酶完全降解植酸的能力，并发现是一种有效的方法。

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