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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment
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Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment

机译：通用异养硝化有氧硝化细菌的氮去除特性，副海桑斯斯DN13-1，海拔深海沉积物分离

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摘要

A heterotrophic nitrifying-aerobic denitrifying bacterium isolated from deep-sea sediment was identified as Pseudomonas bauzanensis DN13-1. Nitrogen (N) removal capability and relative expression of nitrification and denitrification genes of this strain were investigated. The NO2--N, NO3--N and NH4+-N removal efficiencies were 98.82%, 65.87% and 98.89%, respectively, and strain DN13-1 could efficiently remove mixed N. Meanwhile, other inorganic N was not accumulated during these N removal processes. Genomic analysis indicated that genes nirS, norB, nosZ, nasA and putative amo were identified. The relative expression of functional genes by real-time PCR (qPCR) further confirmed nitrite, nitrate and ammonium removal pathways of strain DN13-1 under aerobic condition. Especially, the ammonium removal pathway of this strain was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification directly. Taken together, strain DN13-1 possesses particularity to efficiently remove N, which guarantees its promising application in aquaculture wastewater treatment.
机译：从深海沉积物中分离的异养硝化的有氧硝化细菌被鉴定为假单胞菌Bauzanensis DN13-1。研究了该菌株的氮（N）去除能力和硝化和脱氮基因的相对表达。 NO2 - N，NO3 - N和NH4 + -N去除效率分别为98.82％，65.87％和98.89％，并且菌株DN13-1可以有效地除去混合N.同时，在这些n期间不会累积其他无机N去除过程。基因组分析表明，鉴定了基因NIRS，NORB，NOSZ，NASA和推定的AMO。通过实时PCR（QPCR）通过实时PCR（QPCR）的相对表达进一步证实了有氧条件下菌株DN13-1的亚硝酸盐，硝酸铵和铵去除途径。特别是，通过直接与快速亚硝酸盐脱氮偶联的异养铵硝化达到该菌株的铵去除途径。占据，DN13-1菌株具有特殊性，可以有效地移除N，保证其在水产养殖废水处理中有希望的应用。

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