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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Physico-chemical pretreatment and enzymatic hydrolysis of cotton stalk for ethanol production by Saccharomyces cerevisiae
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Physico-chemical pretreatment and enzymatic hydrolysis of cotton stalk for ethanol production by Saccharomyces cerevisiae

机译：Saccharomyces Cerevisiae的物理化学预处理和纯棉秸秆纯棉秸秆的水解

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The aim of this work was to study the physico-chemical pretreatment and enzymatic hydrolysis of cotton stalk for ethanol production by Saccharomyces cerevisiae. Firstly, factors affecting pretreatment were screened out by Plackett-Burman design (PBD) and most significant factors were further optimized by Box-Behnken design (BBD). As shown by experimental study, most significant factors were FeCl3 concentration (FC), irradiation time (IT) and substrate concentration (SC) affecting pretreatment of cotton stalk among all studied factors. Under optimum conditions of pretreatment FC 0.15 mol/l, IT 20 min and SC 55 g/l, the release of reducing sugar was 6.6 g/l. Hydrolysis of pretreated cotton stalk was done by crude on-site produced enzymes and hydrolysate was concentrated. Ethanol production by Saccharomyces cerevisiae using concentrated cotton stalk hydrolysate was 9.8 g(p)/l, with ethanol yield 0.37 g(p)/g(s) on consumed sugars. The data indicated that microwave FeCl3 pretreated cotton stalk hydrolyses by crude unprocessed enzyme cocktail was good, and ethanol can be produced by fermentation of hydrolysate.
机译：这项工作的目的是研究Saccharomyces Cerevisiae的乙醇生产的棉茎的物理化学预处理和酶水解。首先，通过Plackett-Burman设计（PBD）筛选影响预处理的因素，并且通过Box-Behnken设计（BBD）进一步优化了大多数重要因素。如实验研究所示，大多数重要因素是FECL3浓度（Fc），照射时间（IT）和底物浓度（sc），影响所有研究中的棉花秸秆的预处理。在预处理的最佳条件下Fc 0.15mol / L，它20分钟和Sc 55克/升，还原糖的释放为6.6g / L.预处理棉茎的水解是通过粗产物产生的酶，浓缩水解产物。使用浓缩棉茎水解产物酿酒酵母的乙醇生产为9.8g（p）/ l，乙醇产率为0.37g（p）/ g（p）的糖。该数据表明，微波FECL3预处理的棉茎水解通过粗产物未加工的酶鸡尾酒良好，乙醇可以通过发酵水解产物来制备。

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