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首页> 外文期刊>Biochemical and Biophysical Research Communications >Insights into regulatory characteristics of the promoters of Sericin 1 and Sericin 3 in transgenic silkworms
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Insights into regulatory characteristics of the promoters of Sericin 1 and Sericin 3 in transgenic silkworms

机译：转基因蚕丝蛋白1和丝氨酸3激发子的监管特征见解

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Sericin, produced in the middle silk gland (MSG) of silkworms, is a group of glue proteins that coat and cement silk fibers. Several genes are known to encode sericin, but their spatiotemporal regulation has yet to be fully elucidated. Here, we report in detail the expression profiles of the promoters of two major sericin-coding genes, Sericin 1 (Ser1)and Sericin 3 (Ser3), by analyzing Gal4/UAS transgenic silkworms. We found that UAS-linked EGFP fluorescence in transgenic silkworms driven by Serl-Gal4was detected in only the R3, R4 and R5 regions of MSG starting inday-3 fifth-instar larvae and was continuously expressed until silk gland degradation. In transgenic silkworms driven by Ser3-Gal4, EGFP fluorescence was detected at a low level in the R2 region of MSG since the last day of fifth-instar larvae, and the expression increased during the wandering stages and was continuously detected until silk gland degradation. The molecular detection of EGFP expression in each of the Gal4/UAS transgenic silkworms was consistent with fluorescence observations. These findings reveal clear differences in the regulatory characteristics of the promoters of Ser1 and Ser3 and provide new insights into the regulatory mechanism of the expression of sericin-coding genes. (C) 2019 Elsevier Inc. All rights reserved.
机译：在蚕丝丝腺（MSG）中产生的硅蛋白是涂层和水泥丝纤维的一组胶水蛋白。已知几种基因编码丝裂蛋白，但它们的时空调节尚未完全阐明。这里，通过分析GAL4 / UAS转基因蚕，详细介绍了两个主要血清编码基因的启动子的表达曲线，丝氨酸1（SeriC1）和Sericin 3（Ser3）。我们发现，由MSG的R3，R4和R5区域检测到的Serl-Gal4和MSG的R3，R4和R5区域驱动的转基因蚕的UAS链接EGFP荧光，并连续地表达直至丝囊降解。在由Ser3-Gal4驱动的转基因蚕中，在第五次幼虫的最后一天以来，在MSG的R2区的低水平下检测EGFP荧光，并且在吹扫阶段的表达增加，并连续检测到丝囊降解直至蚕丝降解。在每个Gal4 / UAS转基因蚕中的EGFP表达的分子检测与荧光观察一致。这些研究结果揭示了Ser1和Ser3启动子的调节特征的明显差异，并对血清素编码基因表达的调节机制提供了新的见解。 （c）2019 Elsevier Inc.保留所有权利。

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